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- W45441678 abstract "In vitro fertilization (IVF) treatment outcomes have continued to improve with the implementation of progressive techniques including the introduction of sequential culture media, blastocyst culture, assisted hatching and pre-implantation screening; however, despite overcoming the barriers to conception, and the precise control over so many pre and post conception aspects, the global IVF success rates remain reasonably static (Beck et al., 2010, Das et al., 2009, Gardner and Lane, 1997, Geary, 2006, Handyside et al., 1992, Munne et al., 1993, Palermo et al., 1992). Preterm birth also continues to be an increasing global problem, which exists in IVF even with the best practice shift from multiple embryo transfers to single embryo transfers, resulting in a reduction in multiple gestations (Beck et al., 2010, Vulliemoz, 2012). The presence of microorganisms within the upper genital tract, and those that contaminate the IVF culture system may result in poor quality oocytes, poor quality embryos (possibly due to oocyte DNA fragmentation), early pregnancy loss or preterm birth. IVF procedures offer a unique opportunity for screening biological fluids (ovarian follicular fluid, Fallopian tube washings, peritoneal fluid and endometrial aspirates) that would not otherwise be available due to the invasive nature of the collection procedure. A small number of researchers have reported isolating microorganisms from the IVF culture system, with mixed reports surrounding the significance of such a discovery (Artley et al., 1993, Cottell et al., 1996, Pelzer et al., 2011). The microbiota (microorganisms, their genetic elements and the environment in which they are living) at each of these sites has the potential to be transferred to additional sites by iatrogenic procedures involved in the IVF cycle (Table I)." @default.
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- W45441678 title "The isolation and identification of microorganisms in the reproductive environment: The potential impact on the IVF culture system and on IVF outcomes" @default.
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