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• W45572903 abstract "Event Abstract Back to Event Study of calpain activity with calcium-independent activator and specific substrate Dávid Czégé1*, A. Farkas2, Katalin Halasy3, K. Tarnok1, P. Friedrich2 and Ildiko Vilagi1 1 Department of Physiology and Neurobiology, Eötvös Loránd University, Hungary 2 Institute of Enzimology, BRC, Hungarian Academy of Science, Hungary 3 Department of Anatomy and Histology, Faculty of Veterinary Science, Szent István, Hungary Calpains, the cytoplasmic Ca2+-activated proteases, have been suggested to play an important role in several physiological and pathological neuronal processes. Earlier we have shown that mixture of A and C subdomains of calpastatin, the specific endogenous inhibitor, in vitro activates calpains in a Ca2+-independent way. We hypothesized this complex may have similar effects in actual biological systems. Our aim was to prove that the calpastatin-derived peptides cross the neuronal membrane and specifically activate calpain, and exert an effect on synaptic level. During this activation no other Ca2+-dependent cytoplasmic processes are triggered. We also made effort to verify that calpains are activated during pathophysiological processes, like epileptiform seizures. Experiments were performed on hippocampal rat brain slices. In the electrophysiological experiments alterations in population spike activities in CA1 region were recorded. In morphological experiments, using different fluorophores, the uptake of the activators and enhancement of calpain activity were demonstrated. We showed that the A and C complex get through the membrane and act as a specific activator of calpain, using a calpain-specific substrate. Entrance of the complex increase neuronal excitability without significant effect on LTP development. We also found that epileptiform activity, without severe cell-damage, were able to induce only weak calpain activation. We propose that the activator complex can be used to specifically activate the calpain system apparently without raising cytoplasmic Ca2+ and clarify the cell types with sensitive calpain system. Such an effector may be a useful tool in dissecting intracellular mechanisms involving cellular processes activated by the calpain system. Supported by OTKA (NK60723) Conference: 12th Meeting of the Hungarian Neuroscience Society, Budapest, Hungary, 22 Jan - 24 Jan, 2009. Presentation Type: Poster Presentation Topic: Developmental neurobiology and subcortical functions Citation: Czégé D, Farkas A, Halasy K, Tarnok K, Friedrich P and Vilagi I (2009). Study of calpain activity with calcium-independent activator and specific substrate. Front. Syst. Neurosci. Conference Abstract: 12th Meeting of the Hungarian Neuroscience Society. doi: 10.3389/conf.neuro.01.2009.04.234 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 13 Mar 2009; Published Online: 13 Mar 2009. * Correspondence: Dávid Czégé, Department of Physiology and Neurobiology, Eötvös Loránd University, Budapest, Hungary, felagund@tolkien.hu Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Dávid Czégé A. Farkas Katalin Halasy K. Tarnok P. Friedrich Ildiko Vilagi Google Dávid Czégé A. Farkas Katalin Halasy K. Tarnok P. Friedrich Ildiko Vilagi Google Scholar Dávid Czégé A. Farkas Katalin Halasy K. Tarnok P. Friedrich Ildiko Vilagi PubMed Dávid Czégé A. Farkas Katalin Halasy K. Tarnok P. Friedrich Ildiko Vilagi Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page." @default.
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• W45572903 date "2009-01-01" @default.
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• W45572903 title "Study of calpain activity with calcium-independent activator and specific substrate" @default.
• W45572903 doi "https://doi.org/10.3389/conf.neuro.01.2009.04.234" @default.
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