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- W455787060 abstract "β-hydroxysteroid dehydrogenase/∆ 5-4 isomerase (3β-HSD) is a key enzyme in placental steroidogenesis. To form steroid hormones, pregnenolone requires enzymes 3β- hydroxysteroiddehydrogenase/∆ 5-4 isomerase (3β-HSD), which belongs to the ∆5 and ∆4 steroidogenic path- ways. The created probes will help to identify the molecular basis of enzymes which play a role in steroidogene- sis. Therefore, the objective of this study was to develop probes with cloning the target gene on a eukaryotic vector. Total RNA was isolated and RT-PCR was performed. DNA bands were extracted from agarose gel and purified. 3βHSD-DNAs were transformed on E.coli strain. Vector containing 3βHSD insert were digested with the restriction enzymes NcoI and NotI. In vitro transcription of the DIG-labelled cRNA was carried out by using DIG-RNA labelling mix. In situ hybridization was carried out with the probes. Specific staining was observed in uninucleated trophoblast cells where as negative control was negative. In conclusion, specific probes were gen- erated with cloning the target gene on a eukaryote vector, whic are sitable for detection of steroidogenic enzymes with in situ hybridization." @default.
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- W455787060 date "2010-01-01" @default.
- W455787060 modified "2023-09-23" @default.
- W455787060 title "A key enzyme in placental steroidogenesis: detection of 3β-hydroxysteroid dehydrogenase/Δ5-4 isomerase (3β-HSD)' ın with cloned probes on eukaryotic vector." @default.
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