Matches in SemOpenAlex for { <https://semopenalex.org/work/W45773818> ?p ?o ?g. }
Showing items 1 to 80 of
80
with 100 items per page.
- W45773818 endingPage "40" @default.
- W45773818 startingPage "935" @default.
- W45773818 abstract "GnRH is capable of inducing an increase in cyclic AMP (AMPc) production in the anterior pituitary gland. However, this intracellular messenger is not involved in the mechanisms leading to acute release of gonadotropins. Using anterior pituitary cells in culture, we recently found that AMPc, like GnRH, stimulated the expression of genes encoding for LH and consequently increased the synthesis of LH subunits. To reevaluate the role of AMPc in the secretion of pituitary gonadotropins, we first studied the effects of 8-Br-AMPc, a permeant AMPc analog, on LH release during a 48-hour period. Direct activation of protein kinases A by 8-Br-AMPc substantially stimulated LH release, to values as high as those seen with GnRH under the optimal conditions used in this experiment. However, kinetics were different, with GnRH inducing a rapid rise in LH and 8-Br-AMPc causing a gradual increase after a five-hour lag. We also studied the effects of 8-Br-AMPc on the release of neosynthesized subunits. Pituitary cells were incubated with 35S-Met, and labeled alpha and LH beta polypeptides were recovered in parallel from the cells and medium. We observed that 8-Br-AMPc stimulated not only LH synthesis but also release of newly synthesized LH subunits, in this respect 8-Br-AMPc was more effective than GnRH. After five hours incubation with 1.5 mM 8-Br-AMPc, 95% of the alpha subunit and 40% of the LH beta subunit that had been newly synthesized were released into the medium, whereas the corresponding figures with exposure to 10 nM GnRH were 80% and 15% respectively.(ABSTRACT TRUNCATED AT 250 WORDS)" @default.
- W45773818 created "2016-06-24" @default.
- W45773818 creator A5009396803 @default.
- W45773818 creator A5017332118 @default.
- W45773818 creator A5024175389 @default.
- W45773818 creator A5028548897 @default.
- W45773818 date "1990-11-01" @default.
- W45773818 modified "2023-09-25" @default.
- W45773818 title "[Cyclic AMP: role in the synthesis and release of luteinizing hormone]." @default.
- W45773818 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/2177539" @default.
- W45773818 hasPublicationYear "1990" @default.
- W45773818 type Work @default.
- W45773818 sameAs 45773818 @default.
- W45773818 citedByCount "0" @default.
- W45773818 crossrefType "journal-article" @default.
- W45773818 hasAuthorship W45773818A5009396803 @default.
- W45773818 hasAuthorship W45773818A5017332118 @default.
- W45773818 hasAuthorship W45773818A5024175389 @default.
- W45773818 hasAuthorship W45773818A5028548897 @default.
- W45773818 hasConcept C104292427 @default.
- W45773818 hasConcept C104317684 @default.
- W45773818 hasConcept C105580179 @default.
- W45773818 hasConcept C126322002 @default.
- W45773818 hasConcept C134018914 @default.
- W45773818 hasConcept C163235415 @default.
- W45773818 hasConcept C185592680 @default.
- W45773818 hasConcept C197012351 @default.
- W45773818 hasConcept C25642318 @default.
- W45773818 hasConcept C2777658017 @default.
- W45773818 hasConcept C2778575703 @default.
- W45773818 hasConcept C2778782382 @default.
- W45773818 hasConcept C2778894405 @default.
- W45773818 hasConcept C2779029589 @default.
- W45773818 hasConcept C49039625 @default.
- W45773818 hasConcept C55493867 @default.
- W45773818 hasConcept C71315377 @default.
- W45773818 hasConcept C71924100 @default.
- W45773818 hasConcept C79879829 @default.
- W45773818 hasConcept C86803240 @default.
- W45773818 hasConceptScore W45773818C104292427 @default.
- W45773818 hasConceptScore W45773818C104317684 @default.
- W45773818 hasConceptScore W45773818C105580179 @default.
- W45773818 hasConceptScore W45773818C126322002 @default.
- W45773818 hasConceptScore W45773818C134018914 @default.
- W45773818 hasConceptScore W45773818C163235415 @default.
- W45773818 hasConceptScore W45773818C185592680 @default.
- W45773818 hasConceptScore W45773818C197012351 @default.
- W45773818 hasConceptScore W45773818C25642318 @default.
- W45773818 hasConceptScore W45773818C2777658017 @default.
- W45773818 hasConceptScore W45773818C2778575703 @default.
- W45773818 hasConceptScore W45773818C2778782382 @default.
- W45773818 hasConceptScore W45773818C2778894405 @default.
- W45773818 hasConceptScore W45773818C2779029589 @default.
- W45773818 hasConceptScore W45773818C49039625 @default.
- W45773818 hasConceptScore W45773818C55493867 @default.
- W45773818 hasConceptScore W45773818C71315377 @default.
- W45773818 hasConceptScore W45773818C71924100 @default.
- W45773818 hasConceptScore W45773818C79879829 @default.
- W45773818 hasConceptScore W45773818C86803240 @default.
- W45773818 hasIssue "9" @default.
- W45773818 hasLocation W457738181 @default.
- W45773818 hasOpenAccess W45773818 @default.
- W45773818 hasPrimaryLocation W457738181 @default.
- W45773818 hasRelatedWork W1983366041 @default.
- W45773818 hasRelatedWork W2005363482 @default.
- W45773818 hasRelatedWork W2014174007 @default.
- W45773818 hasRelatedWork W2024553435 @default.
- W45773818 hasRelatedWork W2033755926 @default.
- W45773818 hasRelatedWork W2045160985 @default.
- W45773818 hasRelatedWork W2055041622 @default.
- W45773818 hasRelatedWork W2071237287 @default.
- W45773818 hasRelatedWork W2134976758 @default.
- W45773818 hasRelatedWork W2141504910 @default.
- W45773818 hasVolume "38" @default.
- W45773818 isParatext "false" @default.
- W45773818 isRetracted "false" @default.
- W45773818 magId "45773818" @default.
- W45773818 workType "article" @default.