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- W45888308 abstract "ABSTRACT Unintegrated supercoiled spleen focus-forming virus (SFFV) DNA was extracted from rat cells newly infected with a woolly monkey virus pseudotype of the Lilly-Steeves strain of SFFV. The DNA was linearized by digestion at the unique HindIII endonuclease site, inserted into the plasmid pBR322, and cloned in an approved EK2 host. Six independent clones containing SFFV DNA inserts were isolated and analyzed by restriction endonuclease digestion. One clone had an insert of 5.7 kbp and also contained the same restriction enzyme sites as the in vivo linear DNA except that it lacked a copy of the terminally redundant sequences. Recombinant DNA isolated from this clone was transfected into NIH 3T3 fibroblasts and rescued either by co-transfection of cloned Friend murine leukemia helper virus (F-MuLV) DNA or by superinfection with a non-erythroblastosis-inducing variant of F-MuLV. Inoculation of adult NIH Swiss mice with the virus produced from these transfections induced an erythroleukemia, characterized by splenomegaly, polycythemia and splenic foci, which was identical to that induced by the original F-MuLV pseudotype of SFFV. Also, the SFFV specific gp52 protein was synthesized in the transfected fibroblasts." @default.
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- W45888308 date "1980-01-01" @default.
- W45888308 modified "2023-09-24" @default.
- W45888308 title "CHARACTERIZATION OF MOLECULARLY CLONED SPLEEN FOCUS-FORMING VIRUS DNA" @default.
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- W45888308 doi "https://doi.org/10.1016/b978-0-12-255850-4.50046-9" @default.
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