Matches in SemOpenAlex for { <https://semopenalex.org/work/W45912085> ?p ?o ?g. }
Showing items 1 to 62 of
62
with 100 items per page.
- W45912085 abstract "Insulin receptor has two splice variants which are produced due to alternative splicing of 36 base pair long exon 11. Inclusion and exclusion of this exon in the matured RNA produces two isoforms, B and A respectively. Previously our lab has generated a minigene construct containing exon 10–12 and the surrounding introns of human IR. Deletion mutation of this construct identified a purine rich ISE near the 5′ and an ISS near the 3′ end of intron 10. For accurate identification of these elements linker scanning mutants were created throughout the ISE and ISS. These constructs were transfected into HepG2 cell lines. The ISE mutants suggest the presence of multiple element binding sites in this region. From ISS mutants, a 12 nt long silencer sequence has been identified, deletion or mutation of which caused a constitutive incorporation of exon 11. Sequence analysis of ISE revealed that it contains potential binding sites for hnRNP A1, F and H proteins. An in vitro RNA pull down assay taking portions of the ISE sequence followed by western blot analysis confirms the binding of these proteins to this region. Co transfection of the minigene with hnRNP A1 inhibits and hnRNP F activates the inclusion of exon 11 in HepG2 cells. Co transfection of the minigene with siRNA against hnRNPA1 and F increases and decreases the inclusion of exon 11 respectively. RNA pull down assay with the ISS revealed the binding of CUG-binding protein 1 (CUGBP-1) and SRp20. Co transfection of wild type minigene with CUGBP-1 inhibits the inclusion of exon 11, whereas, mutation of the 12-nt region of the minigene abolishes the effect of CUGBP-1. Surprisingly, co-transfection of the minigene with SRp20 increased the inclusion of exon 11, suggesting that SRp20 might bind to an enhancer element as well. This study identifies novel intronic regulatory elements and reveals the role of hnRNP A1, F and SRp20 as regulatory proteins for IR splicing." @default.
- W45912085 created "2016-06-24" @default.
- W45912085 creator A5010031854 @default.
- W45912085 creator A5084542813 @default.
- W45912085 creator A5086818902 @default.
- W45912085 date "2008-03-01" @default.
- W45912085 modified "2023-10-18" @default.
- W45912085 title "SRp20, hnRNP A1 and F regulate alternative splicing of insulin receptor via binding to the intronic regulatory elements" @default.
- W45912085 doi "https://doi.org/10.1096/fasebj.22.1_supplement.601.1" @default.
- W45912085 hasPublicationYear "2008" @default.
- W45912085 type Work @default.
- W45912085 sameAs 45912085 @default.
- W45912085 citedByCount "1" @default.
- W45912085 crossrefType "journal-article" @default.
- W45912085 hasAuthorship W45912085A5010031854 @default.
- W45912085 hasAuthorship W45912085A5084542813 @default.
- W45912085 hasAuthorship W45912085A5086818902 @default.
- W45912085 hasConcept C104317684 @default.
- W45912085 hasConcept C143065580 @default.
- W45912085 hasConcept C153911025 @default.
- W45912085 hasConcept C166342232 @default.
- W45912085 hasConcept C194583182 @default.
- W45912085 hasConcept C36823959 @default.
- W45912085 hasConcept C41282012 @default.
- W45912085 hasConcept C54009773 @default.
- W45912085 hasConcept C54355233 @default.
- W45912085 hasConcept C54458228 @default.
- W45912085 hasConcept C67705224 @default.
- W45912085 hasConcept C86803240 @default.
- W45912085 hasConcept C94671646 @default.
- W45912085 hasConceptScore W45912085C104317684 @default.
- W45912085 hasConceptScore W45912085C143065580 @default.
- W45912085 hasConceptScore W45912085C153911025 @default.
- W45912085 hasConceptScore W45912085C166342232 @default.
- W45912085 hasConceptScore W45912085C194583182 @default.
- W45912085 hasConceptScore W45912085C36823959 @default.
- W45912085 hasConceptScore W45912085C41282012 @default.
- W45912085 hasConceptScore W45912085C54009773 @default.
- W45912085 hasConceptScore W45912085C54355233 @default.
- W45912085 hasConceptScore W45912085C54458228 @default.
- W45912085 hasConceptScore W45912085C67705224 @default.
- W45912085 hasConceptScore W45912085C86803240 @default.
- W45912085 hasConceptScore W45912085C94671646 @default.
- W45912085 hasIssue "S1" @default.
- W45912085 hasLocation W459120851 @default.
- W45912085 hasOpenAccess W45912085 @default.
- W45912085 hasPrimaryLocation W459120851 @default.
- W45912085 hasRelatedWork W1994924520 @default.
- W45912085 hasRelatedWork W2096708722 @default.
- W45912085 hasRelatedWork W2167154687 @default.
- W45912085 hasRelatedWork W2463093367 @default.
- W45912085 hasRelatedWork W2528442309 @default.
- W45912085 hasRelatedWork W2560858633 @default.
- W45912085 hasRelatedWork W2566893986 @default.
- W45912085 hasRelatedWork W2612230280 @default.
- W45912085 hasRelatedWork W3128134256 @default.
- W45912085 hasRelatedWork W4200277849 @default.
- W45912085 hasVolume "22" @default.
- W45912085 isParatext "false" @default.
- W45912085 isRetracted "false" @default.
- W45912085 magId "45912085" @default.
- W45912085 workType "article" @default.