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- W46079126 abstract "Tobacco ringspot virus (TRSV) and Tomato ringspot virus (ToRSV) are quarantine pests. For the detection of these two viruses, we adopted Trizol for total RNA extraction and developed a semi-nested RT-PCR method with its specific primers, which designed on the basis of the coding genes of TRSV and ToRSV coat protein, respectively. Products of TRSV by RT-PCR and semi-nested PCR were 359 bp and 206 bp, that of ToRSV were 340 bp and 219 bp. Sequencing of the semi-nested RT-PCR products of TRSV and ToRSV indicated that their identities with the TRSV and ToRSV coat protein genes were 88% -97% and 96%-100%, respectively. Further study showed that the detection limits of DAS-ELISA and RT-PCR methods were about the same, but 10~3 times lower than that of the semi-nested RT-PCR at least." @default.
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- W46079126 date "2007-01-01" @default.
- W46079126 modified "2023-09-26" @default.
- W46079126 title "Detection of Tobacco ringspot virus and Tomato ringspot virus by semi-nested RT-PCR." @default.
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