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- W46221431 endingPage "142" @default.
- W46221431 startingPage "138" @default.
- W46221431 abstract "pHZ1276是透明颤菌血红蛋白基因(vgb)的诱导型表达载体,携带有硫链丝菌素诱导的强启动子PtipA和φC31int,attP基因使载体整合在染色体上.本研究利用组成型启动子ermP1P2替代pHZ1276上的诱导型启动子PtipA构建了组成型表达血红蛋白基因的质粒pHZ1291.将vgb基因去掉后构建了组成型链霉菌表达载体pHZ1294.将pHZ1276,pHZ1291导入阿维链霉菌(Streptomyces avermitilis)NRRL8165,所得重组子经Southern杂交验证后,蛋白粗提物经Western杂交和CO结合实验表明vgb基因在该菌中表达出了有活性的VHb蛋白." @default.
- W46221431 created "2016-06-24" @default.
- W46221431 creator A5079566375 @default.
- W46221431 creator A5089700002 @default.
- W46221431 creator A5091513663 @default.
- W46221431 date "2005-03-30" @default.
- W46221431 modified "2023-09-24" @default.
- W46221431 title "透明颤菌血红蛋白基因(vgb)在阿维链霉菌的整合表达" @default.
- W46221431 hasPublicationYear "2005" @default.
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