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- W46256969 abstract "Virus-induced gene silencing using artificial microRNAs (MIR VIGS) is a newly developed technique for plant reverse genetic studies. Traditional virus-induced gene silencing (VIGS) assays introduce a large gene fragment, which is expressed and then converted into small RNAs by the endogenous siRNA-based gene silencing machinery of the plant host. By contrast, MIR VIGS uses well-designed miRNAs to induce RNA-mediated silencing of the target gene. Using a single artificial miRNA can provide greater specificity by reducing off-target effects. Here, we describe a detailed protocol for MIR VIGS in Nicotiana benthamiana using a modified Cabbage leaf curl virus (CaLCuV)-based vector." @default.
- W46256969 created "2016-06-24" @default.
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- W46256969 date "2013-01-01" @default.
- W46256969 modified "2023-09-26" @default.
- W46256969 title "Virus-Induced Gene Silencing Using Artificial miRNAs in Nicotiana benthamiana" @default.
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- W46256969 doi "https://doi.org/10.1007/978-1-62703-278-0_8" @default.
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