Matches in SemOpenAlex for { <https://semopenalex.org/work/W46889237> ?p ?o ?g. }
Showing items 1 to 64 of
64
with 100 items per page.
- W46889237 abstract "The ubiquitin-proteasome pathway is involved in regulation of a variety of biologically important processes including antigen presentation by macrophages (Mφ). Age-related decrease in proteasome activity has been reported in other tissues. However, the effect of aging on the ubiquitin-proteasome pathway in Mφ has not been systematically studied. Thioglycollate-elicited Mφ from young (4–6 mo) and Old (24–26 mo) male C57BL/6NIA mice were collected by lavage. Mφ from old mice exhibited significantly lower activity of all three proteasome peptidases [chymotrypsin-like activity (young vs old, Mean ± SE): 100 ± 13 vs 62 ± 4, p<0.05; trypsin-like activity: 100 ± 4 vs 56 ± 3, p<0.01; acidic activity: 100 ± 7 vs 58 ± 6, p<0.01]. The age-related decrease in proteasome activity was not due to decreased expression of proteasome subunits, since levels of C2 and S1 subunits were higher in old Mφ than young (C2: 100 ± 20 vs 167 ± 13, p<0.05; S1: 100 ± 15 vs 183 ± 20, p<0.01) while there was no significant age-related difference in levels of α5, LMP2 and LMP7. The decrease in proteasome activity was associated with an increase in the levels of ubiquitin conjugates. Furthermore, the levels of 2 ubiquitination-related enzymes, E1 and E2 (Ubc7), were significantly higher in old Mφ than young. The age-related decline in proteasome activity and the paralleled increase in levels of ubiquitin conjugates indicate that the function of the ubiquitin-proteasome pathway is impaired in the aged Mφ. The decrease in proteasome activity in Mφ might contribute to the age-related decline in the ability of Mφ to present antigens to T cells, a proteasome dependent process. Supported by USDA #58-1950-9-001 and NIA #R01 AG009140-10A1." @default.
- W46889237 created "2016-06-24" @default.
- W46889237 creator A5001356589 @default.
- W46889237 creator A5030986047 @default.
- W46889237 creator A5075762269 @default.
- W46889237 creator A5077006581 @default.
- W46889237 date "2006-03-01" @default.
- W46889237 modified "2023-09-26" @default.
- W46889237 title "Proteasome activity declines in aged macrophages" @default.
- W46889237 doi "https://doi.org/10.1096/fasebj.20.5.a983-b" @default.
- W46889237 hasPublicationYear "2006" @default.
- W46889237 type Work @default.
- W46889237 sameAs 46889237 @default.
- W46889237 citedByCount "0" @default.
- W46889237 crossrefType "journal-article" @default.
- W46889237 hasAuthorship W46889237A5001356589 @default.
- W46889237 hasAuthorship W46889237A5030986047 @default.
- W46889237 hasAuthorship W46889237A5075762269 @default.
- W46889237 hasAuthorship W46889237A5077006581 @default.
- W46889237 hasConcept C104317684 @default.
- W46889237 hasConcept C126322002 @default.
- W46889237 hasConcept C134018914 @default.
- W46889237 hasConcept C181199279 @default.
- W46889237 hasConcept C185592680 @default.
- W46889237 hasConcept C25602115 @default.
- W46889237 hasConcept C27740335 @default.
- W46889237 hasConcept C2780340462 @default.
- W46889237 hasConcept C2781307694 @default.
- W46889237 hasConcept C55493867 @default.
- W46889237 hasConcept C71924100 @default.
- W46889237 hasConcept C86803240 @default.
- W46889237 hasConceptScore W46889237C104317684 @default.
- W46889237 hasConceptScore W46889237C126322002 @default.
- W46889237 hasConceptScore W46889237C134018914 @default.
- W46889237 hasConceptScore W46889237C181199279 @default.
- W46889237 hasConceptScore W46889237C185592680 @default.
- W46889237 hasConceptScore W46889237C25602115 @default.
- W46889237 hasConceptScore W46889237C27740335 @default.
- W46889237 hasConceptScore W46889237C2780340462 @default.
- W46889237 hasConceptScore W46889237C2781307694 @default.
- W46889237 hasConceptScore W46889237C55493867 @default.
- W46889237 hasConceptScore W46889237C71924100 @default.
- W46889237 hasConceptScore W46889237C86803240 @default.
- W46889237 hasFunder F4320306114 @default.
- W46889237 hasFunder F4320337337 @default.
- W46889237 hasIssue "5" @default.
- W46889237 hasLocation W468892371 @default.
- W46889237 hasOpenAccess W46889237 @default.
- W46889237 hasPrimaryLocation W468892371 @default.
- W46889237 hasRelatedWork W1583190421 @default.
- W46889237 hasRelatedWork W1996811776 @default.
- W46889237 hasRelatedWork W2029270045 @default.
- W46889237 hasRelatedWork W2045482490 @default.
- W46889237 hasRelatedWork W2050485954 @default.
- W46889237 hasRelatedWork W2063876242 @default.
- W46889237 hasRelatedWork W2086984568 @default.
- W46889237 hasRelatedWork W2201531372 @default.
- W46889237 hasRelatedWork W2409119444 @default.
- W46889237 hasRelatedWork W3010056926 @default.
- W46889237 hasVolume "20" @default.
- W46889237 isParatext "false" @default.
- W46889237 isRetracted "false" @default.
- W46889237 magId "46889237" @default.
- W46889237 workType "article" @default.