Matches in SemOpenAlex for { <https://semopenalex.org/work/W4707788> ?p ?o ?g. }
- W4707788 endingPage "36" @default.
- W4707788 startingPage "2529" @default.
- W4707788 abstract "To investigate the molecular mechanism relating phenotype and genotype in the rd mouse, mRNA and pre-mRNA levels derived from the wild-type and position-347 nonsense mutant beta-phosphodiesterase (beta-PDE) genes were determined and compared with the corresponding gene copy ratios.Total RNA and genomic DNA was isolated from the retinas of three heterozygous rd/+ mouse strains. For each, quantitative reverse transcription-polymerase chain reaction (RT-PCR) was used to determine the ratio of wild-type and rd beta-phosphodiesterase pre-mRNA and mature mRNA. The gene copy ratio between wild-type and rd beta-PDE was also determined by quantitative PCR.The pre-mRNA ratio of wild-type versus nonsense mutant was close to 1:1, whereas the corresponding mRNA ratio was greater than 3:1, even though the gene copy ratio was confirmed to be 1:1.The equivalence of pre-mRNA ratio level for wild-type and nonsense mutant in the rd/+ retina indicates that both genes were transcribed at similar levels. Thus, neither the nonsense mutation at position 347 nor the intron 1 retroviral insertion also present in the rd gene seem to have affected gene transcription. In contrast, the strain-independent bias favoring wild-type mature mRNA in vivo suggests a specific degradation of mutant transcript during or after pre-mRNA splicing. This allele-specific degradation serves to decrease mutant transcript levels dramatically in all rd strains, and suggests that photoreceptor cells have the capacity to reduce the level of an mRNA containing a nonsense mutation." @default.
- W4707788 created "2016-06-24" @default.
- W4707788 creator A5017740223 @default.
- W4707788 creator A5022143096 @default.
- W4707788 creator A5086264524 @default.
- W4707788 date "1998-12-01" @default.
- W4707788 modified "2023-09-23" @default.
- W4707788 title "Selective degradation of nonsense beta-phosphodiesterase mRNA in the heterozygous rd mouse." @default.
- W4707788 cites W101789412 @default.
- W4707788 cites W1514240524 @default.
- W4707788 cites W1536091221 @default.
- W4707788 cites W1562615854 @default.
- W4707788 cites W1566243287 @default.
- W4707788 cites W1576457008 @default.
- W4707788 cites W1761769533 @default.
- W4707788 cites W1916759561 @default.
- W4707788 cites W1964211379 @default.
- W4707788 cites W1964244564 @default.
- W4707788 cites W1965825681 @default.
- W4707788 cites W1967757221 @default.
- W4707788 cites W1975304302 @default.
- W4707788 cites W1987857855 @default.
- W4707788 cites W1991416943 @default.
- W4707788 cites W1992009563 @default.
- W4707788 cites W2012582259 @default.
- W4707788 cites W2026480278 @default.
- W4707788 cites W2027588113 @default.
- W4707788 cites W2027632084 @default.
- W4707788 cites W2027956702 @default.
- W4707788 cites W2032489802 @default.
- W4707788 cites W2040211989 @default.
- W4707788 cites W2041102000 @default.
- W4707788 cites W2041395068 @default.
- W4707788 cites W2043382556 @default.
- W4707788 cites W2046608233 @default.
- W4707788 cites W2052012202 @default.
- W4707788 cites W2062742450 @default.
- W4707788 cites W2069342877 @default.
- W4707788 cites W2076125296 @default.
- W4707788 cites W2082540519 @default.
- W4707788 cites W2086859078 @default.
- W4707788 cites W2092489342 @default.
- W4707788 cites W2092559748 @default.
- W4707788 cites W2093009120 @default.
- W4707788 cites W2094967199 @default.
- W4707788 cites W2099375764 @default.
- W4707788 cites W2117770954 @default.
- W4707788 cites W2127860156 @default.
- W4707788 cites W2131091865 @default.
- W4707788 cites W2140157294 @default.
- W4707788 cites W2143609676 @default.
- W4707788 cites W2146856288 @default.
- W4707788 cites W2156933588 @default.
- W4707788 cites W2409690503 @default.
- W4707788 cites W282739772 @default.
- W4707788 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/9856762" @default.
- W4707788 hasPublicationYear "1998" @default.
- W4707788 type Work @default.
- W4707788 sameAs 4707788 @default.
- W4707788 citedByCount "6" @default.
- W4707788 countsByYear W47077882015 @default.
- W4707788 countsByYear W47077882018 @default.
- W4707788 crossrefType "journal-article" @default.
- W4707788 hasAuthorship W4707788A5017740223 @default.
- W4707788 hasAuthorship W4707788A5022143096 @default.
- W4707788 hasAuthorship W4707788A5086264524 @default.
- W4707788 hasConcept C104317684 @default.
- W4707788 hasConcept C105580179 @default.
- W4707788 hasConcept C138885662 @default.
- W4707788 hasConcept C143065580 @default.
- W4707788 hasConcept C153911025 @default.
- W4707788 hasConcept C179926584 @default.
- W4707788 hasConcept C207583985 @default.
- W4707788 hasConcept C41895202 @default.
- W4707788 hasConcept C501734568 @default.
- W4707788 hasConcept C54355233 @default.
- W4707788 hasConcept C75563809 @default.
- W4707788 hasConcept C86803240 @default.
- W4707788 hasConcept C94671646 @default.
- W4707788 hasConcept C96777560 @default.
- W4707788 hasConceptScore W4707788C104317684 @default.
- W4707788 hasConceptScore W4707788C105580179 @default.
- W4707788 hasConceptScore W4707788C138885662 @default.
- W4707788 hasConceptScore W4707788C143065580 @default.
- W4707788 hasConceptScore W4707788C153911025 @default.
- W4707788 hasConceptScore W4707788C179926584 @default.
- W4707788 hasConceptScore W4707788C207583985 @default.
- W4707788 hasConceptScore W4707788C41895202 @default.
- W4707788 hasConceptScore W4707788C501734568 @default.
- W4707788 hasConceptScore W4707788C54355233 @default.
- W4707788 hasConceptScore W4707788C75563809 @default.
- W4707788 hasConceptScore W4707788C86803240 @default.
- W4707788 hasConceptScore W4707788C94671646 @default.
- W4707788 hasConceptScore W4707788C96777560 @default.
- W4707788 hasIssue "13" @default.
- W4707788 hasLocation W47077881 @default.
- W4707788 hasOpenAccess W4707788 @default.
- W4707788 hasPrimaryLocation W47077881 @default.