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• W47332021 abstract "The transition to flowering is one of the most important development switches in the life of a plant. For some species this switch appears only once in life and is therefore tightly regulated. Flowering is directly coupled to reproductive success and therefore has to occur under optimal conditions. Several genetic pathways regulate the transition to flowering. CO is a central component of the photoperiod pathway and mediates flowering in response to day length by regulating the expression of FT. CO encodes a B-Box transcription factor that also contains a plant specific CCT-domain. Since CO does not contain a known DNA-binding motif I conducted yeast-two-hybrid screening to identify proteins that recruit CO to DNA. In addition, I conducted yeast-one-hybrid screening to identify proteins regulating FT expression. Here I present evidence that CO interacts with all members of the heterotrimeric CCAAT-box-binding factor (CBF). The CBF-complex consists of three subunits named HAP2, HAP3 and HAP5. HAP3 and HAP5 dimerize and associate with the DNA-binding subunit HAP2. Both CCT-domain and HAP2 proteins contain the HAP2-DNA-binding motif. Mutations affecting conserved residues in this domain cause loss-of-function phenotypes in CCT-domain proteins, which might be due to an impaired interaction with HAP2. The HAP3a subunit is co-regulated with CO by GIGANTEA and expression of a putative dominant negative transgene causes late flowering. MtN19 was found to interact with the CCT-domain of CO and with the FT promoter in yeast. Studies on this gene suggest a possible regulation by natural antisense transcription since transgenic plants expressing a dsRNAi construct are late or early flowering. The early flowering lines express MtN19, CO and FT at high levels. Late flowering plants can not be rescued by overexpression of CO by the 35S-promoter. MtN19 and CO are both expressed at the end of the light period in long days implying that they might function together to regulate FT expression. FIDGET (FIT) was isolated by yeast-one-hybrid screening and found to bind the FT promoter. It encodes an APETALA2-like protein. Misexpression of FIT in the phloem, the place where FT is naturally expressed, accelerates the floral transition. Moreover, I present evidence that FIT is expressed in vascular tissue upon UV-light induction. Finally, I show that UV-light is able to accelerate the floral transition. Two other AP2-like proteins delay the floral transition when expressed in the phloem and can also interact with the FT promoter. In a nutshell, this thesis presents the first insight how CO may regulate expression of the floral integrator FT and proposes evidence for a novel flowering time pathway involving stress-induced AP2-like transcription factors." @default.
• W47332021 created "2016-06-24" @default.
• W47332021 creator A5041999111 @default.
• W47332021 date "2006-01-01" @default.
• W47332021 modified "2023-09-23" @default.
• W47332021 title "Analysis of the function of the CONSTANS protein and transcriptional regulation of FLOWERING LOCUS T" @default.
• W47332021 hasPublicationYear "2006" @default.
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