Matches in SemOpenAlex for { <https://semopenalex.org/work/W47459054> ?p ?o ?g. }
Showing items 1 to 78 of
78
with 100 items per page.
- W47459054 endingPage "250" @default.
- W47459054 startingPage "245" @default.
- W47459054 abstract "An HPLC method was developed to determine the plasma concentrations of R(+)- and S(−)-thiopentone for pharmacokinetic studies in sheep. The method required separation of the thiopentone enantiomers from the corresponding pentobarbitone enantiomers which are usually present as metabolites of thiopentone. Phenylbutazone was used as an internal standard. After acidification, the plasma samples were extracted with a mixture of ether and hexane (2:8). The solvent was evaporated to dryness and the residues were reconstituted with sodium hydroxide solution (pH 10). The samples were chromatographed on a 100 mm × 4 mm I.D.. Chiral AGP-CSP column. The mobile phase was 4.5% 2-propanol in 0.1 M phosphate buffer (pH 6.2) with a flow-rate of 0.9 ml/min. This gave k′ values of 1.92, 2.92, 5.71, 9.30 and 11.98 for R(+)-pentobarbitone, S(−)-pentobarbitone, R(+)-thiopentone, S(−)-thiopentone, and phenylbutazone, respectively. At detection wavelength of 287 nm, the limit of quantitation was 5 ng/ml for R(+)-thiopentone and 6 ng/ml for S(−)-thiopentone. The inter-day coefficients of variation at concentrations of 0.02, 0.1 and 8 μg/ml were, respectively, 4.8, 4.4 and 3.5% for R(+)-thiopentone and, respectively, 5.0, 4.3 and 3.9% for S(−)-thiopentone (n = 6 each enantiomer). At the same concentrations, the intra-day coefficients of variation from six sets of replicates (measured over six days) were, respectively, 8.0, 8.0 and 8.8% for R(+)-thiopentene and 8.8, 7.4 and 9.6% for S(−)-thiopentone. Linearity over the standard range, 0.01–40 μg/ml, was shown by correlation coefficients > 0.998. This method has proven suitable for pharmacokinetic studies of thiopentone enantiomers after administration of rac-thiopentone in human plasma also and would be suitable for pharmacokinetic studies of the pentobarbitone eantiomers." @default.
- W47459054 created "2016-06-24" @default.
- W47459054 creator A5029477822 @default.
- W47459054 creator A5030239133 @default.
- W47459054 creator A5076082726 @default.
- W47459054 date "1995-11-01" @default.
- W47459054 modified "2023-09-25" @default.
- W47459054 title "High-performance liquid chromatographic determination of thiopentene enantiomers in sheep plasma" @default.
- W47459054 cites W118739103 @default.
- W47459054 cites W1964018023 @default.
- W47459054 cites W1982111847 @default.
- W47459054 cites W1992941537 @default.
- W47459054 cites W1998548487 @default.
- W47459054 cites W2002890804 @default.
- W47459054 cites W2015010670 @default.
- W47459054 cites W2022617085 @default.
- W47459054 cites W2027538847 @default.
- W47459054 cites W2032129728 @default.
- W47459054 cites W2051783790 @default.
- W47459054 cites W2053480499 @default.
- W47459054 cites W2066609138 @default.
- W47459054 cites W2151958420 @default.
- W47459054 cites W2164774707 @default.
- W47459054 cites W2165856696 @default.
- W47459054 cites W2242387829 @default.
- W47459054 doi "https://doi.org/10.1016/0378-4347(95)00269-6" @default.
- W47459054 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8611958" @default.
- W47459054 hasPublicationYear "1995" @default.
- W47459054 type Work @default.
- W47459054 sameAs 47459054 @default.
- W47459054 citedByCount "19" @default.
- W47459054 countsByYear W474590542016 @default.
- W47459054 crossrefType "journal-article" @default.
- W47459054 hasAuthorship W47459054A5029477822 @default.
- W47459054 hasAuthorship W47459054A5030239133 @default.
- W47459054 hasAuthorship W47459054A5076082726 @default.
- W47459054 hasConcept C147789679 @default.
- W47459054 hasConcept C179998833 @default.
- W47459054 hasConcept C185592680 @default.
- W47459054 hasConcept C2775844104 @default.
- W47459054 hasConcept C2778562268 @default.
- W47459054 hasConcept C43617362 @default.
- W47459054 hasConcept C486523 @default.
- W47459054 hasConcept C71240020 @default.
- W47459054 hasConcept C71924100 @default.
- W47459054 hasConcept C98274493 @default.
- W47459054 hasConceptScore W47459054C147789679 @default.
- W47459054 hasConceptScore W47459054C179998833 @default.
- W47459054 hasConceptScore W47459054C185592680 @default.
- W47459054 hasConceptScore W47459054C2775844104 @default.
- W47459054 hasConceptScore W47459054C2778562268 @default.
- W47459054 hasConceptScore W47459054C43617362 @default.
- W47459054 hasConceptScore W47459054C486523 @default.
- W47459054 hasConceptScore W47459054C71240020 @default.
- W47459054 hasConceptScore W47459054C71924100 @default.
- W47459054 hasConceptScore W47459054C98274493 @default.
- W47459054 hasIssue "2" @default.
- W47459054 hasLocation W474590541 @default.
- W47459054 hasLocation W474590542 @default.
- W47459054 hasOpenAccess W47459054 @default.
- W47459054 hasPrimaryLocation W474590541 @default.
- W47459054 hasRelatedWork W1974764536 @default.
- W47459054 hasRelatedWork W2020084144 @default.
- W47459054 hasRelatedWork W2021792606 @default.
- W47459054 hasRelatedWork W2036826355 @default.
- W47459054 hasRelatedWork W2044623385 @default.
- W47459054 hasRelatedWork W2062121918 @default.
- W47459054 hasRelatedWork W2119552130 @default.
- W47459054 hasRelatedWork W2166263961 @default.
- W47459054 hasRelatedWork W2196125403 @default.
- W47459054 hasRelatedWork W2390873166 @default.
- W47459054 hasVolume "673" @default.
- W47459054 isParatext "false" @default.
- W47459054 isRetracted "false" @default.
- W47459054 magId "47459054" @default.
- W47459054 workType "article" @default.