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- W48083927 abstract "Many projects, such as multiplex-fluorescence in situ hybridization (M-FISH) karyotyping, require the use of relatively large amounts of multiple fluor- or hapten-labeled nucleotides for the preparation of DNA probes. Such a requirement makes these experimental approaches prohibitively expensive for many researchers. The cost of such nucleotides can be reduced approximately 99% by purchasing the chemical precursors, fluor or hapten succinimidyl esters and 5-(3-aminoallyl)-2′-deoxyuridine 5′ triphosphate (AA-dUTP), and performing the simple coupling/purification described here. It is possible to finish four to ten different fluor/hapten dUTP preparations of 2.5 μM scale within a 24 h period. The reagent cost for each preparation ranges from $33–$237 per μM, depending on the fluor/hapten. This laboratory uses such nucleotide preparations to prepare FISH probes by nick translation or PCR amplification." @default.
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- W48083927 date "2000-03-01" @default.
- W48083927 modified "2023-09-27" @default.
- W48083927 title "Simple Method for Preparation of Fluor/Hapten-Labeled dUTP" @default.
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- W48083927 doi "https://doi.org/10.2144/00283st11" @default.
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