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• W48462938 abstract "In this study, we examined the susceptibility of primordial germ cells (PGCs) to malignant transformation using a transgenic mouse model. In this model we used the simian virus 40 (SV40) large tumor antigen (LTA) fused to the promoter of germ cell alkaline phosphatase (GCAP) to specifically direct the oncogenic activity of SV40-LTA to PGCs. Human GCAP is developmentally expressed in primordial germ cells and also from the 2-cell to blastocyst stage of preimplantation development and reappears in trace amounts in the adult testis. Furthermore, GCAP is reexpressed in precancerous stages of testicular germ cell tumors (carcinoma in situ) and also in more differentiated tumors. We analyzed the regulatory sequences in the 5´-flanking region of human GCAP gene which are responsible for its expression in different testicular germ cell tumors to determine the minimal promoter region sufficient for a high and tissue specific expression of GCAP. Three independent transgenic lines were generated using a fragment of 1.7 kb 5´flanking of the GCAP gene as a promoter. RT-PCR analysis showed testis specific expression of LTA in the adults of heterozygous and homozygous transgenic mice in trace amounts. Furthermore, we could detect LTA mRNA in 8.5 dpc prenatal transgenic embryos. However, even after a period of about 20 months, no tumor was observed in any organ of these mice. Using an in vitro approach of analysing differentially expressed genes in GC-1spg cells through suppression subtractive hybridization (SSH), a so far unknown gene which was found to be highly expressed in invasive GC-1spg cells was identified, we named this gene Pfcp (PHD-finger-like chromatin proteins) as the deduced protein sequence shows a domain known as PHD-finger like domain, known to be involved in chromatin binding. The cDNA sequence of mouse Pfcp was isolated and analyzed as well as its genomic structure. The expression pattern of mouse Pfcp and its cellular and subcellular location was determined: screening a mouse cosmid library and a genomic Southern blot showed the pattern of a gene family which consists of about four different murine Pfcp genes. Northern blot analysis showed one transcript at approximately 1.2 kb. The expression of Pfcp in different mouse tissues is ubiquitous. The expression analysis of human PFCP showed an ubiquitous expression as well. Pfcp is localized in the nucleus and binds to chromatin structures in interphase and metaphase of the cell cycle." @default.
• W48462938 created "2016-06-24" @default.
• W48462938 creator A5034979773 @default.
• W48462938 date "2022-02-16" @default.
• W48462938 modified "2023-10-01" @default.
• W48462938 title "The susceptibility of primordial germ cells to malignant transformation and isolation and characterization of members of a new gene family differentially expressed in invasive and non-invasive immortalized male germ cells" @default.
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• W48462938 doi "https://doi.org/10.53846/goediss-90" @default.
• W48462938 hasPublicationYear "2022" @default.
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• W48462938 hasAuthorship W48462938A5034979773 @default.

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