Matches in SemOpenAlex for { <https://semopenalex.org/work/W48722543> ?p ?o ?g. }
Showing items 1 to 99 of
99
with 100 items per page.
- W48722543 endingPage "644" @default.
- W48722543 startingPage "635" @default.
- W48722543 abstract "1. In incubation mixtures containing digitonin-activated or untreated preparations from rat liver, albumin-solubilized bilirubin as the acceptor substrate and (a) UDP-glucuronic acid, (b) UDP-glucose or (c) UDP-xylose as the sugar donor, formation of the following ester glycosides was demonstrated: with (a), bilirubin beta-d-monoglucuronoside, with (b), bilirubin beta-d-monoglucoside and with (c), bilirubin monoxyloside or mixtures of the mono-and di-xyloside. 2. With UDP-glucuronic acid prolonged incubation and variation of the composition of the incubation mixtures yielded equimolar amounts of azodipyrrole (I) and azodipyrrole beta-d-monoglucuronoside (II) after treatment of the incubation mixtures with the diazonium salt of ethyl anthranilate. The azo-derivatives were identified by t.l.c. by reference to known compounds and by the following chemical tests. After ammonolysis the conjugated azo-derivative (II) yielded d-glucuronic acid and the carboxylic acid amide of azodipyrrole, indicating transfer of a glucuronic acid residue to the carboxylic acid groups of bilirubin. The beta-d-configuration of the sugar moiety and binding at C-1 were demonstrated by enzymic hydrolysis tests. 3. Analogous evidence established the structure of the reaction product obtained with UDP-glucose as the sugar donor, as bilirubin beta-d-monoglucoside. 4. With UDP-xylose as the sugar donor xylosyl transfer to the carboxylic acid groups of bilirubin with attachment at C-1 was demonstrated in an analogous way. A beta-d-configuration is considered very likely, but requires confirmation. 5. Monoxyloside formation was predominant at pH7.4, whereas at decreasing pH values increasing fractions of the substrate were converted into the dixyloside. Prolonged incubation, low concentrations of bilirubin and high concentrations of UDP-xylose favoured diconjugate formation. The available evidence supports the synthesis sequence: bilirubin --> bilirubin monoxyloside --> bilirubin dixyloside." @default.
- W48722543 created "2016-06-24" @default.
- W48722543 creator A5036813949 @default.
- W48722543 creator A5060927817 @default.
- W48722543 creator A5079075767 @default.
- W48722543 creator A5082790102 @default.
- W48722543 date "1972-09-01" @default.
- W48722543 modified "2023-10-16" @default.
- W48722543 title "Structures of bilirubin conjugates synthesized <i>in vitro</i> from bilirubin and uridine diphosphate glucuronic acid, uridine diphosphate glucose or uridine diphosphate xylose by preparations from rat liver" @default.
- W48722543 cites W109632361 @default.
- W48722543 cites W1468107655 @default.
- W48722543 cites W1533022006 @default.
- W48722543 cites W153706089 @default.
- W48722543 cites W1738113832 @default.
- W48722543 cites W1968061508 @default.
- W48722543 cites W1979271594 @default.
- W48722543 cites W1981476884 @default.
- W48722543 cites W2024759433 @default.
- W48722543 cites W2084816083 @default.
- W48722543 cites W2120433679 @default.
- W48722543 cites W2237891921 @default.
- W48722543 cites W2257415660 @default.
- W48722543 cites W2317953583 @default.
- W48722543 cites W2401391346 @default.
- W48722543 cites W2402604840 @default.
- W48722543 cites W2403410908 @default.
- W48722543 cites W2409316002 @default.
- W48722543 cites W34026654 @default.
- W48722543 cites W66925781 @default.
- W48722543 cites W76657351 @default.
- W48722543 doi "https://doi.org/10.1042/bj1290635" @default.
- W48722543 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1174165" @default.
- W48722543 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/4658991" @default.
- W48722543 hasPublicationYear "1972" @default.
- W48722543 type Work @default.
- W48722543 sameAs 48722543 @default.
- W48722543 citedByCount "30" @default.
- W48722543 crossrefType "journal-article" @default.
- W48722543 hasAuthorship W48722543A5036813949 @default.
- W48722543 hasAuthorship W48722543A5060927817 @default.
- W48722543 hasAuthorship W48722543A5079075767 @default.
- W48722543 hasAuthorship W48722543A5082790102 @default.
- W48722543 hasBestOaLocation W487225432 @default.
- W48722543 hasConcept C100817775 @default.
- W48722543 hasConcept C104317684 @default.
- W48722543 hasConcept C181199279 @default.
- W48722543 hasConcept C185592680 @default.
- W48722543 hasConcept C2776648616 @default.
- W48722543 hasConcept C2777513400 @default.
- W48722543 hasConcept C2777902142 @default.
- W48722543 hasConcept C2778152042 @default.
- W48722543 hasConcept C2781259782 @default.
- W48722543 hasConcept C2781278898 @default.
- W48722543 hasConcept C55493867 @default.
- W48722543 hasConcept C67705224 @default.
- W48722543 hasConcept C71240020 @default.
- W48722543 hasConcept C71924100 @default.
- W48722543 hasConcept C87644729 @default.
- W48722543 hasConcept C90924648 @default.
- W48722543 hasConceptScore W48722543C100817775 @default.
- W48722543 hasConceptScore W48722543C104317684 @default.
- W48722543 hasConceptScore W48722543C181199279 @default.
- W48722543 hasConceptScore W48722543C185592680 @default.
- W48722543 hasConceptScore W48722543C2776648616 @default.
- W48722543 hasConceptScore W48722543C2777513400 @default.
- W48722543 hasConceptScore W48722543C2777902142 @default.
- W48722543 hasConceptScore W48722543C2778152042 @default.
- W48722543 hasConceptScore W48722543C2781259782 @default.
- W48722543 hasConceptScore W48722543C2781278898 @default.
- W48722543 hasConceptScore W48722543C55493867 @default.
- W48722543 hasConceptScore W48722543C67705224 @default.
- W48722543 hasConceptScore W48722543C71240020 @default.
- W48722543 hasConceptScore W48722543C71924100 @default.
- W48722543 hasConceptScore W48722543C87644729 @default.
- W48722543 hasConceptScore W48722543C90924648 @default.
- W48722543 hasIssue "3" @default.
- W48722543 hasLocation W487225431 @default.
- W48722543 hasLocation W487225432 @default.
- W48722543 hasLocation W487225433 @default.
- W48722543 hasLocation W487225434 @default.
- W48722543 hasOpenAccess W48722543 @default.
- W48722543 hasPrimaryLocation W487225431 @default.
- W48722543 hasRelatedWork W168538707 @default.
- W48722543 hasRelatedWork W1969499600 @default.
- W48722543 hasRelatedWork W1971442177 @default.
- W48722543 hasRelatedWork W1997308825 @default.
- W48722543 hasRelatedWork W2028208383 @default.
- W48722543 hasRelatedWork W2039360218 @default.
- W48722543 hasRelatedWork W2069368540 @default.
- W48722543 hasRelatedWork W2084752647 @default.
- W48722543 hasRelatedWork W2908391700 @default.
- W48722543 hasRelatedWork W2993216648 @default.
- W48722543 hasVolume "129" @default.
- W48722543 isParatext "false" @default.
- W48722543 isRetracted "false" @default.
- W48722543 magId "48722543" @default.
- W48722543 workType "article" @default.