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- W49340284 abstract "Preparation of mineralized tissue specimens for bone-specific staining encompasses a critical sequence of histological techniques that provides visualization of tissue and cellular morphology. Bone specimens are fixed in 10 % neutral-buffered formalin, dehydrated in graded ethanol (EtOH) solutions (and optionally cleared in xylene), infiltrated and embedded in polymethyl methacrylate (methyl methacrylate), classically sliced into 4–10 micrometer (μm) sections, and stained with bone-specific histological stains such as von Kossa (with either nuclear fast red solution counterstain or MacNeal’s tetrachrome counterstain), modified Goldner’s trichrome, and alizarin red S stain. Here, we describe the tissue processing of mineralized mouse bones from dissection to staining for histological analysis by light microscopy." @default.
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- W49340284 date "2014-01-01" @default.
- W49340284 modified "2023-10-18" @default.
- W49340284 title "Tips and Techniques for Processing and Sectioning Undecalcified Murine Bone Specimens" @default.
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- W49340284 doi "https://doi.org/10.1007/978-1-62703-989-5_10" @default.
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