Matches in SemOpenAlex for { <https://semopenalex.org/work/W493655> ?p ?o ?g. }
Showing items 1 to 77 of
77
with 100 items per page.
- W493655 endingPage "177" @default.
- W493655 startingPage "172" @default.
- W493655 abstract "We investigated the ability of an amphipathic oligopeptide to carry a synthetic ds- DNA oligonucleotide inside human cells. The oligonucleotide was designed as a decoy binding site for the transcriptional activator of the methylguanine-DNA methyltransferase (MGMT) gene. The complex oligopeptide and decoy were administered to MCF10A exponentially growing cells, and the uptake was monitored by flow cytometry. After a 1-h exposure, almost all of the MCF10A cells were fluorescent, indicating that all of the cells had been transfected. By increasing the time, the fluorescence intensity per cell rapidly increased to a plateau at the 8-h time point. RT-PCR analysis of the MGMT gene was used as the molecular readout of the intracellular activity of the DNA decoy. MCF10A cells transfected with the oligopeptide/decoy complex showed a strong reduction in MGMT mRNA. Here, we discuss the advantages of using amphipathic oligopeptides as carriers of short DNA sequences." @default.
- W493655 created "2016-06-24" @default.
- W493655 creator A5018078852 @default.
- W493655 creator A5020252530 @default.
- W493655 creator A5024151669 @default.
- W493655 creator A5047428131 @default.
- W493655 creator A5085271923 @default.
- W493655 creator A5091162026 @default.
- W493655 date "2002-01-01" @default.
- W493655 modified "2023-10-17" @default.
- W493655 title "Efficacy of an Amphipathic Oligopeptide to Shuttle and Release a cis-Acting DNA Decoy into Human Cells" @default.
- W493655 cites W2086274688 @default.
- W493655 doi "https://doi.org/10.2144/02321dd03" @default.
- W493655 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/11808690" @default.
- W493655 hasPublicationYear "2002" @default.
- W493655 type Work @default.
- W493655 sameAs 493655 @default.
- W493655 citedByCount "3" @default.
- W493655 countsByYear W4936552012 @default.
- W493655 countsByYear W4936552015 @default.
- W493655 crossrefType "journal-article" @default.
- W493655 hasAuthorship W493655A5018078852 @default.
- W493655 hasAuthorship W493655A5020252530 @default.
- W493655 hasAuthorship W493655A5024151669 @default.
- W493655 hasAuthorship W493655A5047428131 @default.
- W493655 hasAuthorship W493655A5085271923 @default.
- W493655 hasAuthorship W493655A5091162026 @default.
- W493655 hasBestOaLocation W4936551 @default.
- W493655 hasConcept C104317684 @default.
- W493655 hasConcept C129312508 @default.
- W493655 hasConcept C13935069 @default.
- W493655 hasConcept C153911025 @default.
- W493655 hasConcept C170493617 @default.
- W493655 hasConcept C185592680 @default.
- W493655 hasConcept C2779179475 @default.
- W493655 hasConcept C2779281246 @default.
- W493655 hasConcept C54009773 @default.
- W493655 hasConcept C552990157 @default.
- W493655 hasConcept C553184892 @default.
- W493655 hasConcept C55493867 @default.
- W493655 hasConcept C86803240 @default.
- W493655 hasConceptScore W493655C104317684 @default.
- W493655 hasConceptScore W493655C129312508 @default.
- W493655 hasConceptScore W493655C13935069 @default.
- W493655 hasConceptScore W493655C153911025 @default.
- W493655 hasConceptScore W493655C170493617 @default.
- W493655 hasConceptScore W493655C185592680 @default.
- W493655 hasConceptScore W493655C2779179475 @default.
- W493655 hasConceptScore W493655C2779281246 @default.
- W493655 hasConceptScore W493655C54009773 @default.
- W493655 hasConceptScore W493655C552990157 @default.
- W493655 hasConceptScore W493655C553184892 @default.
- W493655 hasConceptScore W493655C55493867 @default.
- W493655 hasConceptScore W493655C86803240 @default.
- W493655 hasIssue "1" @default.
- W493655 hasLocation W4936551 @default.
- W493655 hasLocation W4936552 @default.
- W493655 hasLocation W4936553 @default.
- W493655 hasOpenAccess W493655 @default.
- W493655 hasPrimaryLocation W4936551 @default.
- W493655 hasRelatedWork W1972731290 @default.
- W493655 hasRelatedWork W2358570302 @default.
- W493655 hasRelatedWork W2369377256 @default.
- W493655 hasRelatedWork W2381657842 @default.
- W493655 hasRelatedWork W2384125650 @default.
- W493655 hasRelatedWork W2401067645 @default.
- W493655 hasRelatedWork W2407199152 @default.
- W493655 hasRelatedWork W493655 @default.
- W493655 hasRelatedWork W2186763657 @default.
- W493655 hasRelatedWork W2434806952 @default.
- W493655 hasVolume "32" @default.
- W493655 isParatext "false" @default.
- W493655 isRetracted "false" @default.
- W493655 magId "493655" @default.
- W493655 workType "article" @default.