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- W49419492 abstract "Somatic hybridization in plants has classically relied on en masse fusion of protoplasts. In general, large populations of protoplasts isolated from both parental forms are mixed and subjected to fusogenic treatments. Most fusion methods require the use of populations in the range of 106 plant cells per experiment. In protoplast fusion in a mixed population all possible combinations of fusants can be generated. In addition, since protoplast fusion frequencies are in the percentage range — depending on the fusogenic treatment used — unfused parental protoplasts will be present in such a mixture. Therefore, protoplast mass fusion allows for only limited, if any, control of the number and type of protoplasts participating in a certain fusion event. Thus, most experiments in plant cell hybridization, following this approach, require the use of selection procedures to enrich for the desired heteroplasmic fusion products out of the unfused parental cells and the homoplasmic fusants." @default.
- W49419492 created "2016-06-24" @default.
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- W49419492 date "1994-01-01" @default.
- W49419492 modified "2023-09-30" @default.
- W49419492 title "Somatic Hybridization by Microfusion of Protoplasts" @default.
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- W49419492 doi "https://doi.org/10.1007/978-3-642-57945-5_3" @default.
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