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- W49519407 abstract "The isocratic retention of 67 widely-different solutes in reversed-phase liquid chromatography (RP-LC) has been investigated as a function of temperature and mobile phase composition (% B) for three different C18 columns. Similar studies were also carried out in a gradient mode, where temperature, gradient time and solvent type were varied. These results show that changes in retention with these conditions are similar for each of these three columns. This suggests that relative column selectivity as defined by experiments for one set of experimental conditions will be approximately applicable for other conditions, with the exception of changes in mobile phase pH—which can affect values of the column parameter C (a measure of silanol ionization). Column selectivity as a function of pH was explored for several columns." @default.
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- W49519407 date "2002-07-01" @default.
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- W49519407 title "Column selectivity in reversed-phase liquid chromatography" @default.
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- W49519407 doi "https://doi.org/10.1016/s0021-9673(02)00660-x" @default.
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