FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W49556117> ?p ?o ?g. }

• W49556117 endingPage "856" @default.
• W49556117 startingPage "851" @default.
• W49556117 abstract "The Se-dependent expression of two selenoproteins, cytosolic glutathione peroxidase (cGPx) and type I iodothyronine-5'-deiodinase (5'DI), was investigated in the porcine epithelial kidney cell line LLC-PK1 in serum-free medium. The selenite-dependent expression of cGPx and 5'DI was revealed by enzyme-activity measurements, affinity labelling of 5'DI, metabolic labelling of proteins with 75Se and steady-state mRNA analysis. The expression of the two enzymes strongly depended on selenite concentrations of the culture medium. cGPx required 2-fold higher selenite levels than 5'DI to reach half-maximal activity. The Se-dependent enzyme activities were approximately paralleled by the corresponding steady-state mRNA levels. The response of the two enzymes to Se supply was further characterized by kinetic Se-depletion and -repletion experiments. Upon removal of medium selenite, cGPx activity decreased exponentially, whereas after an initial decrease over 1-2 days, 5'DI levels completely recovered during a further 2 days. These data indicate a differential Se-dependent regulation of the two selenoproteins, with 5'DI being preferentially supplied with the trace element Se, thus ensuring a continuous cellular capacity for thyroid-hormone activation, even under Se-deficient conditions. The abundant cGPx in cells with sufficient Se supply might serve as a cellular Se store which can be mobilized for the synthesis of more vital selenoproteins such as 5'DI under shortage conditions. Thus, a cellular hierarchy of selenoprotein expression, reflected by different individual regulation mechanisms at the transcriptional and post-transcriptional level, adds to the previously recognized tissue-specific hierarchy of Se retention." @default.
• W49556117 created "2016-06-24" @default.
• W49556117 creator A5002972015 @default.
• W49556117 creator A5021721465 @default.
• W49556117 creator A5052536282 @default.
• W49556117 date "1995-03-15" @default.
• W49556117 modified "2023-10-05" @default.
• W49556117 title "Differential selenium-dependent expression of type I 5′-deiodinase and glutathione peroxidase in the porcine epithelial kidney cell line LLC-PK1" @default.
• W49556117 cites W1208971950 @default.
• W49556117 cites W1490519503 @default.
• W49556117 cites W1506594192 @default.
• W49556117 cites W1525800573 @default.
• W49556117 cites W1529804537 @default.
• W49556117 cites W1537948347 @default.
• W49556117 cites W1542538950 @default.
• W49556117 cites W1545965226 @default.
• W49556117 cites W1548456834 @default.
• W49556117 cites W1563709336 @default.
• W49556117 cites W1568909088 @default.
• W49556117 cites W1571092378 @default.
• W49556117 cites W1572963091 @default.
• W49556117 cites W1580766481 @default.
• W49556117 cites W1591337250 @default.
• W49556117 cites W1597415571 @default.
• W49556117 cites W1604789505 @default.
• W49556117 cites W1641384127 @default.
• W49556117 cites W1904507659 @default.
• W49556117 cites W1966597352 @default.
• W49556117 cites W1968498341 @default.
• W49556117 cites W1969239709 @default.
• W49556117 cites W1969391229 @default.
• W49556117 cites W1970944100 @default.
• W49556117 cites W1985325364 @default.
• W49556117 cites W1987005612 @default.
• W49556117 cites W1990065167 @default.
• W49556117 cites W1997685804 @default.
• W49556117 cites W1999010068 @default.
• W49556117 cites W2001585879 @default.
• W49556117 cites W2002209113 @default.
• W49556117 cites W2012928526 @default.
• W49556117 cites W2020732230 @default.
• W49556117 cites W2036019166 @default.
• W49556117 cites W2039983438 @default.
• W49556117 cites W2042207947 @default.
• W49556117 cites W2052753565 @default.
• W49556117 cites W2058021594 @default.
• W49556117 cites W2059988795 @default.
• W49556117 cites W2060194897 @default.
• W49556117 cites W2060333964 @default.
• W49556117 cites W2060450410 @default.
• W49556117 cites W2067864809 @default.
• W49556117 cites W2068630495 @default.
• W49556117 cites W2073347694 @default.
• W49556117 cites W2077580619 @default.
• W49556117 cites W2083414945 @default.
• W49556117 cites W2084514870 @default.
• W49556117 cites W2109745238 @default.
• W49556117 cites W2126273434 @default.
• W49556117 cites W2128635872 @default.
• W49556117 cites W2135057047 @default.
• W49556117 cites W2144695067 @default.
• W49556117 cites W2148645673 @default.
• W49556117 cites W2168912171 @default.
• W49556117 cites W2175532913 @default.
• W49556117 cites W2215298876 @default.
• W49556117 cites W2410369482 @default.
• W49556117 cites W2421407055 @default.
• W49556117 cites W4241213872 @default.
• W49556117 cites W2322027612 @default.
• W49556117 doi "https://doi.org/10.1042/bj3060851" @default.
• W49556117 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1136598" @default.
• W49556117 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/7702583" @default.
• W49556117 hasPublicationYear "1995" @default.
• W49556117 type Work @default.
• W49556117 sameAs 49556117 @default.
• W49556117 citedByCount "80" @default.
• W49556117 countsByYear W495561172012 @default.
• W49556117 countsByYear W495561172013 @default.
• W49556117 countsByYear W495561172014 @default.
• W49556117 countsByYear W495561172015 @default.
• W49556117 countsByYear W495561172016 @default.
• W49556117 countsByYear W495561172017 @default.
• W49556117 countsByYear W495561172020 @default.
• W49556117 countsByYear W495561172022 @default.
• W49556117 countsByYear W495561172023 @default.
• W49556117 crossrefType "journal-article" @default.
• W49556117 hasAuthorship W49556117A5002972015 @default.
• W49556117 hasAuthorship W49556117A5021721465 @default.
• W49556117 hasAuthorship W49556117A5052536282 @default.
• W49556117 hasBestOaLocation W495561172 @default.
• W49556117 hasConcept C104317684 @default.
• W49556117 hasConcept C105580179 @default.
• W49556117 hasConcept C134018914 @default.
• W49556117 hasConcept C150194340 @default.
• W49556117 hasConcept C153911025 @default.
• W49556117 hasConcept C162008176 @default.
• W49556117 hasConcept C171269688 @default.
• W49556117 hasConcept C178790620 @default.

• next