Matches in SemOpenAlex for { <https://semopenalex.org/work/W49713821> ?p ?o ?g. }
Showing items 1 to 73 of
73
with 100 items per page.
- W49713821 endingPage "109" @default.
- W49713821 startingPage "101" @default.
- W49713821 abstract "High-performance liquid chromatography (HPLC) provides a rapid, sensitive, and reproducible means of separating and quantifying simultaneously a variety of sulfur-containing amino acids and related derivatives. The HPLC method described in this chapter is modified and is based on the initial formation of S-carboxy-methyl derivatives of free thiols followed by the conversion of free amino groups to 2,4-dinitrophenyl (DNP) derivatives. Following derivatization, nanomole levels of individual sulfur-containing amino acids are measured using UV detection at 365 nm after separation by reverse-phase ion-exchange HPLC. Because of the versatility of this HPLC method, biological specimen preparation as well as derivatization and HPLC analysis procedures are discussed. DNP derivatives of acidic amino acids (including thiol-containing compounds) are separated on a 3-aminopropyl column by reversed-phase ion-exchange HPLC. In the mobile phase, methanol is used to elute rapidly the excess 2,4-dinitrophenol and the DNP derivatives of basic and neutral amino acids. Acetic acid is present in the mobile phase to maintain the bonded-phase amino groups in the protonated form. By increasing the sodium acetate concentration of the mobile phase, selective elution of acidic DNP derivatives is accomplished. The eluted DNP derivatives are measured by detection at 365 nm." @default.
- W49713821 created "2016-06-24" @default.
- W49713821 creator A5015649768 @default.
- W49713821 creator A5030798385 @default.
- W49713821 date "1987-01-01" @default.
- W49713821 modified "2023-10-18" @default.
- W49713821 title "High-performance liquid chromatography of thiols and disulfides: Dinitrophenol derivatives" @default.
- W49713821 cites W1511717231 @default.
- W49713821 cites W1699447737 @default.
- W49713821 cites W1970749702 @default.
- W49713821 cites W2013237962 @default.
- W49713821 cites W2072640227 @default.
- W49713821 cites W2078214319 @default.
- W49713821 doi "https://doi.org/10.1016/0076-6879(87)43018-8" @default.
- W49713821 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/3657520" @default.
- W49713821 hasPublicationYear "1987" @default.
- W49713821 type Work @default.
- W49713821 sameAs 49713821 @default.
- W49713821 citedByCount "475" @default.
- W49713821 countsByYear W497138212012 @default.
- W49713821 countsByYear W497138212013 @default.
- W49713821 countsByYear W497138212014 @default.
- W49713821 countsByYear W497138212016 @default.
- W49713821 countsByYear W497138212017 @default.
- W49713821 countsByYear W497138212018 @default.
- W49713821 countsByYear W497138212019 @default.
- W49713821 countsByYear W497138212020 @default.
- W49713821 countsByYear W497138212021 @default.
- W49713821 countsByYear W497138212022 @default.
- W49713821 countsByYear W497138212023 @default.
- W49713821 crossrefType "book-chapter" @default.
- W49713821 hasAuthorship W49713821A5015649768 @default.
- W49713821 hasAuthorship W49713821A5030798385 @default.
- W49713821 hasConcept C178790620 @default.
- W49713821 hasConcept C179998833 @default.
- W49713821 hasConcept C185592680 @default.
- W49713821 hasConcept C2776673659 @default.
- W49713821 hasConcept C2776923573 @default.
- W49713821 hasConcept C2777130120 @default.
- W49713821 hasConcept C43617362 @default.
- W49713821 hasConcept C515207424 @default.
- W49713821 hasConcept C55493867 @default.
- W49713821 hasConcept C73826308 @default.
- W49713821 hasConceptScore W49713821C178790620 @default.
- W49713821 hasConceptScore W49713821C179998833 @default.
- W49713821 hasConceptScore W49713821C185592680 @default.
- W49713821 hasConceptScore W49713821C2776673659 @default.
- W49713821 hasConceptScore W49713821C2776923573 @default.
- W49713821 hasConceptScore W49713821C2777130120 @default.
- W49713821 hasConceptScore W49713821C43617362 @default.
- W49713821 hasConceptScore W49713821C515207424 @default.
- W49713821 hasConceptScore W49713821C55493867 @default.
- W49713821 hasConceptScore W49713821C73826308 @default.
- W49713821 hasLocation W497138211 @default.
- W49713821 hasLocation W497138212 @default.
- W49713821 hasOpenAccess W49713821 @default.
- W49713821 hasPrimaryLocation W497138211 @default.
- W49713821 hasRelatedWork W1908510719 @default.
- W49713821 hasRelatedWork W1970318669 @default.
- W49713821 hasRelatedWork W2001116851 @default.
- W49713821 hasRelatedWork W2048793552 @default.
- W49713821 hasRelatedWork W2065377794 @default.
- W49713821 hasRelatedWork W2086107997 @default.
- W49713821 hasRelatedWork W2126785382 @default.
- W49713821 hasRelatedWork W2149906371 @default.
- W49713821 hasRelatedWork W2389869476 @default.
- W49713821 hasRelatedWork W2946273783 @default.
- W49713821 isParatext "false" @default.
- W49713821 isRetracted "false" @default.
- W49713821 magId "49713821" @default.
- W49713821 workType "book-chapter" @default.