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- W49774080 abstract "To determine the degree of blood cell contamination in GC preparations; to assess techniques of GC purification; and to determine possible effects of contaminating cells and purification techniques on cultured GC in terms of steroid hormone production. Contamination of GC by white blood cells was assessed by Wright’s stain and immunohistochemistry. Purification was attempted by: (1) Ficoll density centrifugation (to remove polymorphonuclear leukocytes [PML]); (2) incubation in tissue culture plastic dishes (to remove adherent monocyte/macrophages); and (3) incubation in the presence of high salt (to remove lymphocytes). Ficoll density centrifugation reduced PML to 2% of total cells, incubation in plastic dishes reduced monocyte/macrophage contamination from 6% to 7% down to <1%, and high-salt incubation reduced lymphocyte contamination from 10% to 12% down to 4%. Granulosa cells plated after preparation with addition of high salt showed increased progesterone production, which could not be entirely explained by the removal of contaminating lymphocytes. These results indicate that the human GC culture system is more complex than is often assumed, and methods that remove a majority of these white blood cells are presented." @default.
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- W49774080 date "1991-11-01" @default.
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- W49774080 title "Human ovarian granulosa cell culture: determination of blood cell contamination and evaluation of possible culture purification steps" @default.
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- W49774080 doi "https://doi.org/10.1016/s0015-0282(16)54659-7" @default.
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