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- W50005597 abstract "Rats were immunized with cells from carcinomas of the urinary bladder, which had been induced in BALB/c mice with 3-methylcholanthrene, and the rat spleens were hybridized with NS-1 mouse myeloma cells. Five of the hybridomas isolated were further studied. One hybridoma, 2H5, defines a protein antigen of 140, 000 molecular weight which is strongly expressed by most chemically induced mouse bladder carcinomas. A second hybridoma, IE6, makes an antibody to an antigen which is expressed by bladder carcinomas but also present in certain normal tissues although in smaller amounts. A third hybridoma, 33E7C, makes an antibody to a glycolipid antigen strongly expressed in bladder carcinoma and weakly expressed elsewhere. A fourth hybridoma, 3B12, makes an antibody to an antigen observed only on bladder carcinomas. The antigens defined by these four hybridomas can be detected at the surface of cultured bladder carcinoma cells by immunofluoresence and also by studying frozen sections of bladder carcinoma using a modification of the Sternberger peroxidase-anti-peroxidase technique. A fifth hybridoma, IG5, defines a cytoskeletal protein which is present in the cytoplasm of both normal and neoplastic bladder epithelium but is much more prevalent in the tumors." @default.
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- W50005597 date "1983-01-01" @default.
- W50005597 modified "2023-10-01" @default.
- W50005597 title "Monoclonal Antibodies to Cell Surface Antigens of Mouse Bladder Carcinomas" @default.
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- W50005597 doi "https://doi.org/10.1016/b978-0-08-029815-3.50076-3" @default.
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