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- W50008828 abstract "The resurrection of the Sleeping Beauty (SB) transposon from molecularly extinct salmonid transposons at the end of last century opened the door for mouse geneticists to develop various transposon-based genetic tool kits, which had already been proven instrumental in Drosophila and other invertebrate model organisms. Since then, transposon technologies have been successfully applied to many aspects of functional genomics, in combination with various well-established tools of mouse genetics including transgenesis and gene targeting. In the SB system, a substantial fraction of the transposition events occurs on the same chromosome, predominantly within 3–4 megabases, while the remainder occurs between different chromosomes in a genome-wide manner. By taking advantage of the two types of transposition, we have developed applications of the SB system for genome-wide mutagenesis as well as region-specific functional analysis of the mouse genome." @default.
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- W50008828 date "2010-01-01" @default.
- W50008828 modified "2023-10-17" @default.
- W50008828 title "Functional Genomics in the Mouse using the Sleeping Beauty Transposon System" @default.
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- W50008828 doi "https://doi.org/10.1016/s0076-6879(10)77005-1" @default.
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