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- W50028239 abstract "The apical Na/K/2Cl cotransporter NKCC2 mediates NaCl reabsorption by the thick ascending limb (TAL). We found that the free radical superoxide stimulates NaCl absorption by TALs by stimulating NKCC2 activity. NKCC2 activity is a product of the number of transporters in the apical membrane plus the rate of turnover of each transporter. We hypothesized that superoxide stimulates apical surface NKCC2 expression. Apical surface NKCC2 expression was measured in rat TAL suspensions by surface biotinylation and Western blot. We found that increasing superoxide production with 1 μM xanthine oxidase and 500 μM hypoxanthine stimulated surface NKCC2 expression by 18±5% (p<0.05). This was blocked by scavenging superoxide with 50 μM tempol. However scavenging hydrogen peroxide with 100 U/mL catalase failed to block superoxide stimulated expression (22±8% of control, p<0.05). Inhibiting endogenous nitric oxide production with L-NAME enhanced the superoxide stimulated surface NKCC2 expression from 21±6 to 41±10% (p<0.05). This effect was completely blocked by scavenging superoxide with 300 U/mL superoxide dismutase. Total NKCC2 expression was not changed by any treatment. We conclude that superoxide increases apical surface NKCC2 expression and this effect is blunted by endogenous nitric oxide. Our data suggest that superoxide stimulates NaCl absorption in part by enhanced trafficking of NKCC2 which may be involved in salt-sensitive hypertension." @default.
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- W50028239 date "2011-04-01" @default.
- W50028239 modified "2023-09-27" @default.
- W50028239 title "SUPEROXIDE INCREASES SURFACE NKCC2 EXPRESSION IN RAT THICK ASCENDING LIMBS" @default.
- W50028239 doi "https://doi.org/10.1096/fasebj.25.1_supplement.1041.20" @default.
- W50028239 hasPublicationYear "2011" @default.
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