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• W50051891 abstract "Research Article1 January 1990free access The U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C (III) in vivo and in vitro. A. Moenne A. Moenne Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author S. Camier S. Camier Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author G. Anderson G. Anderson Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author F. Margottin F. Margottin Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author J. Beggs J. Beggs Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author A. Sentenac A. Sentenac Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author A. Moenne A. Moenne Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author S. Camier S. Camier Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author G. Anderson G. Anderson Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author F. Margottin F. Margottin Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author J. Beggs J. Beggs Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author A. Sentenac A. Sentenac Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. Search for more papers by this author Author Information A. Moenne1, S. Camier1, G. Anderson1, F. Margottin1, J. Beggs1 and A. Sentenac1 1Département de Biologie, CEN de Saclay, Commissariat à l'Energie Atomique, Gif sur Yvette, France. The EMBO Journal (1990)9:271-277https://doi.org/10.1002/j.1460-2075.1990.tb08105.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Unlike the majority of genes encoding small nuclear RNAs, which are transcribed by RNA polymerase B, the U6 gene contains features found in both class B and class C genes, indicating the involvement of a combination of transcription factors normally specific to each class of genes. We present direct genetic and biochemical evidence that the U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C in vivo as well as in vitro. A mutant strain with a temperature-sensitive defect in the large subunit of RNA polymerase C that results in defective transcription of tRNA and 5S RNA genes shows a corresponding defect in U6 RNA levels. Also, purified RNA polymerase C transcribes the U6 gene when supplemented with partially purified TFIIIB. The other class C transcription factors, TFIIIA and Tau (TFIIIC), are not required in this system. Previous ArticleNext Article Volume 9Issue 11 January 1990In this issue RelatedDetailsLoading ..." @default.
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• W50051891 title "The U6 gene of Saccharomyces cerevisiae is transcribed by RNA polymerase C (III) in vivo and in vitro." @default.
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