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- W50108757 abstract "Our laboratory has shown that certain Acid Sensing Ion Channels (ASIC) mediate pressure-induced constriction and migration of vascular smooth muscle cells (VSMC). However, electrophysiological evidence of ASIC channels in VSMC is lacking. The purpose of this study was to test the hypothesis that isolated cerebral artery VSMC express ASIC-like channels. Conventional whole-cell patch-clamp technique was used. The criteria of Na+ selectivity and gating by extracellular acidosis, features of most ASIC channels, were used to confirm the presence of ASIC-like channels. We found extracellular acid induced inward currents in 46% of cells tested (n=126, pH 6.5 -5.0). At pH = 6.0, the mean amplitude of the current normalized to whole cell capacitance was 7.6±0.9 pA/pF (n=22). While initial and sustained responses to acidosis varied among cells, the ASIC blocker amiloride (100 mM) consistently inhibited the initial response (n = 18, pH 6.0-6.5). Extracellular acidosis (pH = 6.0) caused depolarization of 7.2±2.4 mV from the resting membrane potential of 25.4±4.1 mV (n = 8) and shifted the whole-cell reversal potential toward the Nernst potential of Na+ (n = 6). Substitution of extracellular Na+ by N-methyl-d-glucamine abolished the inward current (n = 8), indicating Na+ is the major charge carrier. These findings suggest that functional ASIC-like currents are present in cerebral VSMCs. Supported by the-NHLBI." @default.
- W50108757 created "2016-06-24" @default.
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- W50108757 date "2009-04-01" @default.
- W50108757 modified "2023-09-24" @default.
- W50108757 title "Extracellular acidosis activates ASIC‐like channels in freshly isolated cerebral artery smooth muscle cells" @default.
- W50108757 doi "https://doi.org/10.1096/fasebj.23.1_supplement.1018.13" @default.
- W50108757 hasPublicationYear "2009" @default.
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