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- W5015268 abstract "Synthetic biologists assemble genetic circuits from standardized biological parts called BioBricks™. BioBrick™ examples include promoters, ribosome binding sites, DNA or RNA-coding sequences, and transcriptional terminators. Standard BioBrick™ assembly normally involves assembly of two BioBricks™ at a time using restriction enzymes and DNA ligase. Here we describe an alternative BioBrick™ assembly protocol that describes the assembly of two BioBricks™ using the In-Fusion PCR Cloning Kit. This protocol can also be adapted to use similar recombination-based assembly methods, such as SLIC and Gibson assembly." @default.
- W5015268 created "2016-06-24" @default.
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- W5015268 date "2013-01-01" @default.
- W5015268 modified "2023-09-27" @default.
- W5015268 title "BioBrick™ Assembly Using the In-Fusion PCR Cloning Kit" @default.
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- W5015268 doi "https://doi.org/10.1007/978-1-62703-625-2_3" @default.
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