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- W50164538 abstract "Evaluation of a GC-MS method using N-tert-butyldimethylsilyl-N-methyltrifluoroacetamide (MTBSTFA) as the silylating agent for GC-MS. Study of the stability of creatine and guanidinoacetate in urine. 22 urines were kept at RT, 4 °C and − 30 °C for 15 days. MTBSTFA produces a single chromatographic peak in contrast with other derivatizing agents. Creatine concentration increases at room temperature (326% on average), and at 4 °C (75%). However, detection decreases after freezing (− 37%). Guanidinoacetate is stable, but decreases after freezing (− 37%). Sonication before analysis is crucial to obtain repetitive results. A modified GC-MS method has been validated and the conditions for preservation of the urine have been established." @default.
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- W50164538 date "2009-01-01" @default.
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- W50164538 title "Determination of creatine and guanidinoacetate by GC-MS" @default.
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- W50164538 doi "https://doi.org/10.1016/j.clinbiochem.2008.10.001" @default.
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