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- W50448074 abstract "To achieve high-throughput analysis of allele frequencies in human SNPs, we have developed automated methods for designing PCR and DNA sequencing primers. We found we could run the PCR assays at quite stringent, uniform conditions. The design process used freely available databases, including dbSNP, SNPper, and TSC, and publicly available software including RepeatMasker and Primer3. We describe parameters for the software and other considerations that increase experimental success. As anticipated, some assays failed at the design stage due primarily to the genomic locations of repetitive sequences, extreme GC content regions, or lack of sufficient sequence. However, over 23 000 assays, including 96% of those recently analyzed, have been experimentally successful. Similar design methods could be used for PCR assays in any organism with substantial available sequence." @default.
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- W50448074 date "2002-06-01" @default.
- W50448074 modified "2023-10-03" @default.
- W50448074 title "Primer Design for PCR and Sequencing in High-Throughput Analysis of SNPs" @default.
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- W50448074 doi "https://doi.org/10.2144/jun0202" @default.
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