Matches in SemOpenAlex for { <https://semopenalex.org/work/W50458077> ?p ?o ?g. }
Showing items 1 to 69 of
69
with 100 items per page.
- W50458077 abstract "DNA double strand breaks (DSBs) can cause massive genome instability. In eukaryotes, multiple pathways exist to resolve DNA DSBs. One major repair pathway, homologous recombination (HR), utilizes homologous DNA templates to mediate repair. The presence of repetitive elements in the template DNA used for repair can lead to mutagenic repair. Prior studies in Saccharomyces cerevisiae have observed gross chromosomal rearrangements (GCRs) mediated by repetitive DNA from the Ty1 retrotransposon family. Ty1 retrotransposons are highly repetitive sequence elements located throughout the S. cerevisaie genome. In this work, we utilize an endogenous Ty1 element to investigate Ty1- mediated GCRs. We show that the presence of an endogenous high copy repeat sequence in a nonessential chromosome arm increases the rate of GCR formation involving that chromosome arm by nearly 400 fold. Almost all GCRs isolated in this study were mediated by this high copy repeat sequence and ranged from simple monocentric nonreciprocal translocations to nonreciprocal translocations involving template switches to breakage- fusion-bridge (BFB) events. Additionally, despite different mutational defects, the nonreciprocal translocation phenotype appeared to be the predominant GCR phenotype. By adapting a previously developed PCR technique known as multiplex ligation-dependent amplification (MLPA) to study the distribution profiles of GCRs formed in both wild type and mutant strains, we identify ectopic high copy repeat target hotspots. These hotspots mediate the majority of the observed recombinations in wild type strains and may act as fragile sites in the genome. Surprisingly, we find examples of high copy repeat-mediated nonreciprocal translocations in a mutant deficient for RAD52, an important HR gene. Finally, we demonstrate that loss of a histone H3 lysine residue 56 (H3K56) acetylator leads to an increased frequency of aneuploidy that occurs simultaneously with observed GCR events. Taken together, the results indicate Ty1 repeat sequences greatly increase genome instability and that the mechanisms causing increased instability can be further explored using our adapted MLPA technique" @default.
- W50458077 created "2016-06-24" @default.
- W50458077 creator A5041842073 @default.
- W50458077 date "2011-01-01" @default.
- W50458077 modified "2023-09-27" @default.
- W50458077 title "Effects of a highly repetitive endogenous Ty1 retrotransposon on genome stability" @default.
- W50458077 hasPublicationYear "2011" @default.
- W50458077 type Work @default.
- W50458077 sameAs 50458077 @default.
- W50458077 citedByCount "0" @default.
- W50458077 crossrefType "journal-article" @default.
- W50458077 hasAuthorship W50458077A5041842073 @default.
- W50458077 hasConcept C101434241 @default.
- W50458077 hasConcept C102744134 @default.
- W50458077 hasConcept C104317684 @default.
- W50458077 hasConcept C138626823 @default.
- W50458077 hasConcept C141231307 @default.
- W50458077 hasConcept C143425029 @default.
- W50458077 hasConcept C156695909 @default.
- W50458077 hasConcept C178169997 @default.
- W50458077 hasConcept C199491388 @default.
- W50458077 hasConcept C4918238 @default.
- W50458077 hasConcept C50919411 @default.
- W50458077 hasConcept C54355233 @default.
- W50458077 hasConcept C552990157 @default.
- W50458077 hasConcept C7029365 @default.
- W50458077 hasConcept C86803240 @default.
- W50458077 hasConceptScore W50458077C101434241 @default.
- W50458077 hasConceptScore W50458077C102744134 @default.
- W50458077 hasConceptScore W50458077C104317684 @default.
- W50458077 hasConceptScore W50458077C138626823 @default.
- W50458077 hasConceptScore W50458077C141231307 @default.
- W50458077 hasConceptScore W50458077C143425029 @default.
- W50458077 hasConceptScore W50458077C156695909 @default.
- W50458077 hasConceptScore W50458077C178169997 @default.
- W50458077 hasConceptScore W50458077C199491388 @default.
- W50458077 hasConceptScore W50458077C4918238 @default.
- W50458077 hasConceptScore W50458077C50919411 @default.
- W50458077 hasConceptScore W50458077C54355233 @default.
- W50458077 hasConceptScore W50458077C552990157 @default.
- W50458077 hasConceptScore W50458077C7029365 @default.
- W50458077 hasConceptScore W50458077C86803240 @default.
- W50458077 hasLocation W504580771 @default.
- W50458077 hasOpenAccess W50458077 @default.
- W50458077 hasPrimaryLocation W504580771 @default.
- W50458077 hasRelatedWork W1720377166 @default.
- W50458077 hasRelatedWork W1975057042 @default.
- W50458077 hasRelatedWork W1996877626 @default.
- W50458077 hasRelatedWork W2037969252 @default.
- W50458077 hasRelatedWork W2041120137 @default.
- W50458077 hasRelatedWork W2049857374 @default.
- W50458077 hasRelatedWork W2050194148 @default.
- W50458077 hasRelatedWork W2063294632 @default.
- W50458077 hasRelatedWork W2080812640 @default.
- W50458077 hasRelatedWork W2098932435 @default.
- W50458077 hasRelatedWork W2104951427 @default.
- W50458077 hasRelatedWork W2108953735 @default.
- W50458077 hasRelatedWork W2114255733 @default.
- W50458077 hasRelatedWork W2136223090 @default.
- W50458077 hasRelatedWork W2151198423 @default.
- W50458077 hasRelatedWork W2159629613 @default.
- W50458077 hasRelatedWork W2165859227 @default.
- W50458077 hasRelatedWork W2796235790 @default.
- W50458077 hasRelatedWork W2903527054 @default.
- W50458077 hasRelatedWork W3047826374 @default.
- W50458077 isParatext "false" @default.
- W50458077 isRetracted "false" @default.
- W50458077 magId "50458077" @default.
- W50458077 workType "article" @default.