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• W50496774 abstract "Research Article1 October 1992free access DNA transcription and repressor binding affect deletion formation in Escherichia coli plasmids. D. Vilette D. Vilette Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author M. Uzest M. Uzest Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author S.D. Ehrlich S.D. Ehrlich Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author B. Michel B. Michel Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author D. Vilette D. Vilette Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author M. Uzest M. Uzest Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author S.D. Ehrlich S.D. Ehrlich Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author B. Michel B. Michel Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. Search for more papers by this author Author Information D. Vilette1, M. Uzest1, S.D. Ehrlich1 and B. Michel1 1Laboratoire de Génétique Microbienne, Institut National de la Recherche Agronomique, Jouy en Josas, France. The EMBO Journal (1992)11:3629-3634https://doi.org/10.1002/j.1460-2075.1992.tb05447.x PDFDownload PDF of article text and main figures. ToolsAdd to favoritesDownload CitationsTrack CitationsPermissions ShareFacebookTwitterLinked InMendeleyWechatReddit Figures & Info Chimeric plasmids containing phage M13 and plasmid pBR322 sequences undergo deletions in Escherichia coli with a high frequency. In all plasmids one deletion endpoint is the M13 replication origin nick site. We examined the effects of transcription on the position of the other deletion end-point, by inserting in the plasmids an inducible promoter followed by a transcription terminator. Transcription dramatically affected deletions in an orientation-dependent way, such that greater than 95% of end-points were localized downstream from the inserted promoter when it faced the major plasmid transcripts. The end-points were not constrained to the transcribed region and were not affected by the orientation of pBR322 DNA replication. We propose that deletion events occur preferentially in a plasmid domain which is rendered positively supercoiled by convergent transcription. We also show that interaction of LacI repressor with the cognate operator generates a localized deletion hot spot. This hot spot is dependent on pBR322 replication, and therefore probably acts by arresting progression of DNA replication. Previous ArticleNext Article Volume 11Issue 101 October 1992In this issue RelatedDetailsLoading ..." @default.
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• W50496774 title "DNA transcription and repressor binding affect deletion formation in Escherichia coli plasmids." @default.
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