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- W50958873 abstract "FGD5 is a putative Rho family guanine nucleotide exchange factor enriched in vascular endothelial cells (EC). We observed FGD5 expression in human EC and seek to determine FGD5 function. Inhibition of FGD5 expression using RNAi decreased the protein by ~70%. In 3D angiogenesis in vitro, FGD5-knockdown (kd) EC had 45 +/− 8% reduced sprout outgrowth and sprout length versus non-silenced (NS) controls. We examined FGD5-kd EC adhesion to matrix. FGD5-kd cell adhesion to fibronectin was reduced by 28 +/− 4% vs. NS. Similarly, monolayer electrical impedance was decreased after seeding FGD5-kd cells versus controls, and impedance increased at slower rate (4.6 +/− 0.6 siNS vs. 2.4 +/− 0.5 siFGD5 Ohm/min) reflecting decreased EC spreading. Apoptosis was examined by measurement of cleaved caspase-3 expression and subdiploid DNA content. After pro-apoptotic cyclohexamide and tumor necrosis factor-α stimulus, FGD5-kd markedly increased apoptosis (54 +/− 4% vs 34 +/− 8% EC display active caspase-3 and 54 +/− 4% vs 29 +/− 3% EC have subdipolid DNA among siFGD5 vs siNS-treated cells respectively). These results indicate that FGD5 in EC is involved in adhesion, survival, and angiogenesis." @default.
- W50958873 created "2016-06-24" @default.
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- W50958873 date "2012-04-01" @default.
- W50958873 modified "2023-09-23" @default.
- W50958873 title "FGD5 deficiency confers defective matrix adhesion and survival among endothelial cells" @default.
- W50958873 doi "https://doi.org/10.1096/fasebj.26.1_supplement.55.8" @default.
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