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- W51323828 abstract "Abstract Regulation of immune responses and tolerance is mediated by a variety of mechanisms and cells. So far the role of B cells in this process is not well known, yet. Methods: Highly purified CD19+B and CD4+T cells were separated from human PBMC by MACS. B cells were prestimulated with SAC, αIgM/IgG or αCD40, washed and set up in cocultures with freshly isolated autologous CD4+T cells under addition of αCD3+IL-2 or αCD28. CD4+ T cell proliferation was determined after 3–6d by 3H Thymidine incorporation and PKH-26; apoptosis by AnnexinV. Results: under optimal stimulatory conditions T cell proliferation was inhibited more than 50% in presence of SAC-activated B cells and to a lesser extent by αIgM/IgG stimulated B cells. Enrichment of SAC-activated large CD25+ B cells by FACSsorting enhanced suppression further up to 68%, while the small CD25− population had no significant effect. Separation of B and T cells by cell culture inserts abolished inhibition, suggesting a requirement for direct cell-contact. In addition to growth inhibition, T cell specific cytokine production (IFNγ, IL-10) was downregulated, and a significant proportion of T cells (37% vs 17%) went into apoptosis. Conclusions: B cells are able to inhibit T cell mediated responses depending on their mode of activation. Further experiments are dealing with the mechanisms of B cell mediated suppression and their pathophysiological impact also in autoimmune diseases." @default.
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- W51323828 date "2007-04-01" @default.
- W51323828 modified "2023-09-27" @default.
- W51323828 title "Identification of human B cells with immunoregulatory properties (92.12)" @default.
- W51323828 doi "https://doi.org/10.4049/jimmunol.178.supp.92.12" @default.
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