FRINK Linked Data Fragments server

Matches in SemOpenAlex for { <https://semopenalex.org/work/W51328637> ?p ?o ?g. }

• W51328637 endingPage "73" @default.
• W51328637 startingPage "268" @default.
• W51328637 abstract "To investigate the effect of Der f 1 mRNA molecules for specific immunotherapy on murine model of asthma.Fifty BALB/c mice were randomly divided into 5 groups: PBS group, Der f 1 sensitization group, Der f 1 specific immunotherapy (SIT) group, beta-actin mRNA SIT group, and Derf 1 mRNA SIT group. On days 0, 7 and 14, mice in PBS group received PBS injection; mice in the other groups were intraperitoneally injected with 10 microg Derf 1. At day 21, the mice in the 4 experimental groups were challenged with a 30-min inhaled dose of Der f 1 (100 microg/ml) for 7 successive days. Two weeks after the final sensitization, the mice in the above five groups were im- munized by intradermal injection with PBS, 1 microg Der f 1, 10 microg Der f 1, 2 microg beta-actin mRNA, and 2 microg Der f 1 mRNA, respectively for 3 times at one-week intervals. Two weeks after the last intradermal injection, all mice were sacrificed and bronchoalveolar lavage fluid (BALF) was collected. ELISA was performed to detect the levels of IFN-gamma and IL-13 in BALF, the number of eosinophils in the BALF was recorded. Splenocytes were prepared, and cultured with Der f 1 al- lergen (10 Jg/ml) for 72 h. Splenocytes of PBS group was cultured without Derf 1 allergen. The levels of IFN-gamma and IL-13 in splenocyte culture supernatant were measured by ELISA, as well as serum antibody levels of total IgE, allergen- specific IgE (sIgE), sIgG1, and sIgG2a. Lung sections were stained in hematoxylin and eosin, and observed under the microsope.Except for PBS group, mice in the other 4 group showed symptoms of acute asthma attack. Com- pared with Derf 1 sensitization group [(897.56 +/- 105.73) pg/ml] and beta-actin mRNA SIT group [(219.47 +/- 64.72) pg/ml], the level of IFN-gamma in BALF from Der f 1 mRNA SIT group [(897.56 +/- 105.73) pg/ml] and Derfl SIT group [(864.48 +/- 70.62)pg/ml] significantly increased (P<0.01). However, the level of IL-13 in BALF from Derf 1 mRNA SIT group [(241.64 +/- 31.41) pg/ml] and Derf 1 SIT group [(321.94 +/- 41.07)pg/ml] was significantly lower than that of Der f 1 sensitization group [(520.62 +/- 43.77) pg/ml] and beta-actin mRNA SIT group [(507.22 +/- 42.26) pg/ml](P<0.01). The number of eosinophils in Der f 1 mRNA SIT group [(1.33 +/- 0.44) x 10(5)/ml] and Der f 1 SIT group [(1.48 +/- 0.39) x 10(5)/ml] was also lower than that of Der f 1 sensitization group [(3.54 +/- 0.52)x10(5)/ml] and beta-actin mRNA SIT group [(2.98-0.53) x 10(5)/ml] (P<0.01). The levels of IFN-GAMMA and IL-13 in splenocyte culture supernatant showed that IFN-gamma level in Der f 1 mRNA SIT group [(420.91+69.92) pg/ml] and Der f 1 SIT group [(334.92 +/- 43.72) pg/ml] was significantly higher than that of Der f 1 sensitization group[(123.75 +/- 5.48) pg/ml] and beta-actin mRNA SIT group[(128.84 +/- 59.00) pg/ml] (P<0.01). However, IL-13 level of Der f 1 mRNA SIT group [(268.51 +/- 40.42) pg/ml] and Der f 1 SIT group [(285.26 +/- 62.21) pg/ml] was significantly lower than that of Derf 1 sensitization group [(613.89 +/- 51.54) pg/ml] and beta-actin mRNA SIT group [(524.05 +/- 39.12) pg/ml] (P<0.01). Compared with Der f 1 sensitization group [total IgE: (94.34 +/- 11.66) ng/ml, sIgE: (65.67 +/- 9.47) ng/ml, sIgG1: (75.18 +/- 9.52) ng/ml, sIgG2a: (2.81 +/- 1.17) ng/ml] and beta-actin mRNA SIT group[total IgE: (86.48 +/- 10.26) ng/ml, sIgE: (62.36 +/- 8.35) ng/ml, sIgG1: (69.51 +/- 8.98) ng/ml, IgG2a: (1.06 +/- 0.11) ng/ml], the serum antibody levels of total IgE [(33.72 +/- 9.78) ng/ml], sIgE [(22.76 +/- 8.09) ng/ml], sIgG1 [(17.87 +/- 7.59) ng/ml] of Der f 1 mRNA SIT group decreased significantly (P<0.01), whereas the level of IgG% [(7.74 +/- 0.88) ng/ml] increased (P<0.01). Compared with Der f 1 sensitization group, the asthmatic symptoms were relieved after immunization with Der f 1 mRNA for specific immunotherapy, including intact structure of respiratory and alveolar epithelial cells, decreased inflammatory cell infiltration, and similar to those in Der f 1 SIT group. However, the breakage and detachment of bronchial epithelial cells occurred in beta-actin mRNA SIT group.Derf 1 mRNA vaccine can correct Th1 and Th2 imbalance." @default.
• W51328637 created "2016-06-24" @default.
• W51328637 creator A5009514449 @default.
• W51328637 creator A5029169082 @default.
• W51328637 creator A5046708957 @default.
• W51328637 creator A5066286002 @default.
• W51328637 creator A5070055073 @default.
• W51328637 date "2014-08-01" @default.
• W51328637 modified "2023-10-14" @default.
• W51328637 title "[Experimental study on the Der f 1 mRNA molecules derived from dermatophagoides farinae for specific immunotherapy on murine model of asthma]." @default.
• W51328637 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/25518589" @default.
• W51328637 hasPublicationYear "2014" @default.
• W51328637 type Work @default.
• W51328637 sameAs 51328637 @default.
• W51328637 citedByCount "0" @default.
• W51328637 crossrefType "journal-article" @default.
• W51328637 hasAuthorship W51328637A5009514449 @default.
• W51328637 hasAuthorship W51328637A5029169082 @default.
• W51328637 hasAuthorship W51328637A5046708957 @default.
• W51328637 hasAuthorship W51328637A5066286002 @default.
• W51328637 hasAuthorship W51328637A5070055073 @default.
• W51328637 hasConcept C104317684 @default.
• W51328637 hasConcept C105580179 @default.
• W51328637 hasConcept C126322002 @default.
• W51328637 hasConcept C141105273 @default.
• W51328637 hasConcept C153911025 @default.
• W51328637 hasConcept C159654299 @default.
• W51328637 hasConcept C16685009 @default.
• W51328637 hasConcept C185592680 @default.
• W51328637 hasConcept C189976730 @default.
• W51328637 hasConcept C203014093 @default.
• W51328637 hasConcept C207480886 @default.
• W51328637 hasConcept C2776217527 @default.
• W51328637 hasConcept C2777701055 @default.
• W51328637 hasConcept C2777714996 @default.
• W51328637 hasConcept C2777961210 @default.
• W51328637 hasConcept C2780510475 @default.
• W51328637 hasConcept C55493867 @default.
• W51328637 hasConcept C71924100 @default.
• W51328637 hasConcept C86803240 @default.
• W51328637 hasConcept C8891405 @default.
• W51328637 hasConceptScore W51328637C104317684 @default.
• W51328637 hasConceptScore W51328637C105580179 @default.
• W51328637 hasConceptScore W51328637C126322002 @default.
• W51328637 hasConceptScore W51328637C141105273 @default.
• W51328637 hasConceptScore W51328637C153911025 @default.
• W51328637 hasConceptScore W51328637C159654299 @default.
• W51328637 hasConceptScore W51328637C16685009 @default.
• W51328637 hasConceptScore W51328637C185592680 @default.
• W51328637 hasConceptScore W51328637C189976730 @default.
• W51328637 hasConceptScore W51328637C203014093 @default.
• W51328637 hasConceptScore W51328637C207480886 @default.
• W51328637 hasConceptScore W51328637C2776217527 @default.
• W51328637 hasConceptScore W51328637C2777701055 @default.
• W51328637 hasConceptScore W51328637C2777714996 @default.
• W51328637 hasConceptScore W51328637C2777961210 @default.
• W51328637 hasConceptScore W51328637C2780510475 @default.
• W51328637 hasConceptScore W51328637C55493867 @default.
• W51328637 hasConceptScore W51328637C71924100 @default.
• W51328637 hasConceptScore W51328637C86803240 @default.
• W51328637 hasConceptScore W51328637C8891405 @default.
• W51328637 hasIssue "4" @default.
• W51328637 hasLocation W513286371 @default.
• W51328637 hasOpenAccess W51328637 @default.
• W51328637 hasPrimaryLocation W513286371 @default.
• W51328637 hasRelatedWork W2029245561 @default.
• W51328637 hasRelatedWork W2044944420 @default.
• W51328637 hasRelatedWork W2067824418 @default.
• W51328637 hasRelatedWork W2136747657 @default.
• W51328637 hasRelatedWork W2140674811 @default.
• W51328637 hasRelatedWork W2166050584 @default.
• W51328637 hasRelatedWork W2166925955 @default.
• W51328637 hasRelatedWork W2327017460 @default.
• W51328637 hasRelatedWork W2555839509 @default.
• W51328637 hasRelatedWork W2752683031 @default.
• W51328637 hasVolume "32" @default.
• W51328637 isParatext "false" @default.
• W51328637 isRetracted "false" @default.
• W51328637 magId "51328637" @default.
• W51328637 workType "article" @default.