Matches in SemOpenAlex for { <https://semopenalex.org/work/W52166051> ?p ?o ?g. }
Showing items 1 to 95 of
95
with 100 items per page.
- W52166051 endingPage "68" @default.
- W52166051 startingPage "68" @default.
- W52166051 abstract "In mammals, the surface glycoproteins, CD4 and CD8, define different functional subset of T lymphocytes and may act as recognition molecules mediating appropriate interactions between T cells and their targets. The chicken represents an excellent model to study the development of both T and B lymphocytes, since each popUlation develops in a distinct organ, i. e. the thymus and bursa respectively. The development and functions of chicken T lymphocytes however, have not been investigated as extensively as in mammals. Recently in this laboratory, the monoclonal antibodies Lc-4 and Lc-6 have been established, recogniz ing molecules equivalent to mammalian CDS and CD4 antigens respectively, based on their tissue distribution and biochemical properties. In this study, the cloning of chicken CD4 and CDS genes was attempted using these monoclonal antibodies. A cDNA library was constructed with the CDM8 ex pression vector from chicken thymocytes, poly (A)-containing RNA, by the method of Aruffo and Seed, introduced into COS 1 cells, and then the cells expressing the Lc-6 antigen were isolated by cell sorting with the monoclonal antibody Lc-6. The cDNAs of these positive cells were then extracted by the method of Hirt and reintroduced to Esherichia coli. The colonies were screened by cDNA probes prepared from MSBl cells, which belong to chicken lymphoblastoid cell line derived from Marek's disease. The cDNAs of colonies which hybridized with the MSBl probes were introduced into COSl cells, allowing the expression of Lc-6 antigen to be determined by indirect immunofluorescence. One out of thirteen transfectant clones was positive. The size of the DNA insert in this clone was L 9 kbp. This gene, encoding the Lc-6 antigen, was shown not to be rearranged by genomic blot analysis. The sequencing of the DNA from this clone is now in progress. An attempt to clone the Lc-4 antigen coding gene using the same cloning procedures was unsuccessful. The cloning and analysis of genes coding T cell antigens should contribute to the understanding of the evolution of the immune system of the chicken." @default.
- W52166051 created "2016-06-24" @default.
- W52166051 creator A5056127027 @default.
- W52166051 date "1991-05-30" @default.
- W52166051 modified "2023-09-23" @default.
- W52166051 title "MOLECULAR CLONING OF CHICKEN T LYMPHOCYTE SURFACE ANTIGENS" @default.
- W52166051 hasPublicationYear "1991" @default.
- W52166051 type Work @default.
- W52166051 sameAs 52166051 @default.
- W52166051 citedByCount "0" @default.
- W52166051 crossrefType "journal-article" @default.
- W52166051 hasAuthorship W52166051A5056127027 @default.
- W52166051 hasConcept C104317684 @default.
- W52166051 hasConcept C108625454 @default.
- W52166051 hasConcept C121050878 @default.
- W52166051 hasConcept C144024400 @default.
- W52166051 hasConcept C147483822 @default.
- W52166051 hasConcept C149923435 @default.
- W52166051 hasConcept C153911025 @default.
- W52166051 hasConcept C159654299 @default.
- W52166051 hasConcept C167672396 @default.
- W52166051 hasConcept C187882448 @default.
- W52166051 hasConcept C189482833 @default.
- W52166051 hasConcept C195616568 @default.
- W52166051 hasConcept C199360897 @default.
- W52166051 hasConcept C203014093 @default.
- W52166051 hasConcept C2776090121 @default.
- W52166051 hasConcept C2777187973 @default.
- W52166051 hasConcept C2778236600 @default.
- W52166051 hasConcept C2778630268 @default.
- W52166051 hasConcept C2908647359 @default.
- W52166051 hasConcept C2992881475 @default.
- W52166051 hasConcept C41008148 @default.
- W52166051 hasConcept C542903549 @default.
- W52166051 hasConcept C54355233 @default.
- W52166051 hasConcept C553184892 @default.
- W52166051 hasConcept C86803240 @default.
- W52166051 hasConcept C8891405 @default.
- W52166051 hasConceptScore W52166051C104317684 @default.
- W52166051 hasConceptScore W52166051C108625454 @default.
- W52166051 hasConceptScore W52166051C121050878 @default.
- W52166051 hasConceptScore W52166051C144024400 @default.
- W52166051 hasConceptScore W52166051C147483822 @default.
- W52166051 hasConceptScore W52166051C149923435 @default.
- W52166051 hasConceptScore W52166051C153911025 @default.
- W52166051 hasConceptScore W52166051C159654299 @default.
- W52166051 hasConceptScore W52166051C167672396 @default.
- W52166051 hasConceptScore W52166051C187882448 @default.
- W52166051 hasConceptScore W52166051C189482833 @default.
- W52166051 hasConceptScore W52166051C195616568 @default.
- W52166051 hasConceptScore W52166051C199360897 @default.
- W52166051 hasConceptScore W52166051C203014093 @default.
- W52166051 hasConceptScore W52166051C2776090121 @default.
- W52166051 hasConceptScore W52166051C2777187973 @default.
- W52166051 hasConceptScore W52166051C2778236600 @default.
- W52166051 hasConceptScore W52166051C2778630268 @default.
- W52166051 hasConceptScore W52166051C2908647359 @default.
- W52166051 hasConceptScore W52166051C2992881475 @default.
- W52166051 hasConceptScore W52166051C41008148 @default.
- W52166051 hasConceptScore W52166051C542903549 @default.
- W52166051 hasConceptScore W52166051C54355233 @default.
- W52166051 hasConceptScore W52166051C553184892 @default.
- W52166051 hasConceptScore W52166051C86803240 @default.
- W52166051 hasConceptScore W52166051C8891405 @default.
- W52166051 hasIssue "1" @default.
- W52166051 hasLocation W521660511 @default.
- W52166051 hasOpenAccess W52166051 @default.
- W52166051 hasPrimaryLocation W521660511 @default.
- W52166051 hasRelatedWork W146246930 @default.
- W52166051 hasRelatedWork W1551039287 @default.
- W52166051 hasRelatedWork W1974560975 @default.
- W52166051 hasRelatedWork W1990225212 @default.
- W52166051 hasRelatedWork W1993833167 @default.
- W52166051 hasRelatedWork W2015724580 @default.
- W52166051 hasRelatedWork W2102003861 @default.
- W52166051 hasRelatedWork W2154166376 @default.
- W52166051 hasRelatedWork W2352394988 @default.
- W52166051 hasRelatedWork W2352485553 @default.
- W52166051 hasRelatedWork W2354236014 @default.
- W52166051 hasRelatedWork W2356297234 @default.
- W52166051 hasRelatedWork W2364359800 @default.
- W52166051 hasRelatedWork W2366981525 @default.
- W52166051 hasRelatedWork W2373139272 @default.
- W52166051 hasRelatedWork W2374900234 @default.
- W52166051 hasRelatedWork W2377830445 @default.
- W52166051 hasRelatedWork W2381310994 @default.
- W52166051 hasRelatedWork W2402737111 @default.
- W52166051 hasRelatedWork W2865670941 @default.
- W52166051 hasVolume "39" @default.
- W52166051 isParatext "false" @default.
- W52166051 isRetracted "false" @default.
- W52166051 magId "52166051" @default.
- W52166051 workType "article" @default.