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- W525612 abstract "This chapter discusses the assay of phosphatase activity by continuous measurements, which has the advantage that only one pipetting is necessary and the measurements can be carried out rapidly. The amount of 4-nitrophenol liberated is measured continuously at 405 nm. Serum alkaline phosphatase is most active in 2 M diethanolamine buffer, pH 9.8 with 25 mM nitrophenylphosphate. Alterations in temperature give considerable variations in the extinctions (possibly because of differences in substrate dissociation). The buffer/substrate solution is stable for 8 days at 0–4°C. If the measurements cannot be made at 405 nm, a standard curve must be prepared. With values around 300 U/l, the coefficient of variation in series of assays was 3.9%. According to studies, the normal range for adults of 18–85 years is 66–230 U/l. To determine alkaline phosphatase activity in leucocyte suspensions, the leucocytes are isolated from freshly collected blood, repeatedly washed, and suspended in physiological NaCl. The phosphatase activity in tissue homogenates has usually been measured with glycerophosphate as substrate." @default.
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- W525612 date "1974-01-01" @default.
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- W525612 title "Alkaline Phosphatase in Serum" @default.
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