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- W53006449 abstract "Abstract: Our laboratory has been constructing plasmids with suitable genetic properties for the cloning of DNA fragments. We have combined the essential replication properties of the plasmid ColEl with two antibiotic resistance markers (tetracycline and ampicillin) to generate a series of related plasmids. During the course of the construction of these plasmids we have determined the positions of restriction sites used for cloning DNA fragments relative to the antibiotic resistance genes and the origin of DNA replication. We have mapped a number of restriction sites on a series of related plasmids. We will summarize the development of two plasmids, pBR313 and pBR322, which are useful cloning vehicles for in vitro recombinant DNA research. The characterization of these plasmids has prompted an investigation of the nature of tetracycline resistance determined by the plasmid pSC101 and the pMB9 series of plasmids." @default.
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- W53006449 date "1977-01-01" @default.
- W53006449 modified "2023-09-25" @default.
- W53006449 title "CHARACTERIZATION OF TETRACYCLINE AND AMPICILLIN RESISTANT PLASMID CLONING VEHICLES" @default.
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- W53006449 doi "https://doi.org/10.1016/b978-0-12-634250-5.50009-4" @default.
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