Matches in SemOpenAlex for { <https://semopenalex.org/work/W53008384> ?p ?o ?g. }
Showing items 1 to 93 of
93
with 100 items per page.
- W53008384 abstract "Many arylamine and hydrazine drugs and xenobiotics are acetylated by liver N-acetyltransferase (NAT; EC 2.3.1.5). Two loci, mnat and pnat, encode the enzymes designated monomorphic and polymorphic NAT (mNAT and pNAT) respectively. These isoenzymes have different substrate specificities. In addition, at the polymorphic locus a diversity of alleles is found, which differ by specific point mutations that may or may not result in amino acid substitutions. These point mutations result in the ‘slow’ acetylation of substrates of pNAT. The substrates for NAT include carcinogenic arylamines. Susceptibility to bladder cancer has been related to slow acetylation. NAT has been characterized in immortalized human cell lines to assess their use in studies of the metabolism or arylamines in vitro. A monocytic cell line (U937) and two hepatoma cell lines of parenchymal lineage (HepG2 and Hep3B) have been shown to catalyse acetylation of substrates of mNAT but do not acetylate sulphamethazine, a substrate specific for pNAT. Using PCR to amplify the alleles of pNAT, followed by restriction-enzyme digestion of the product, the cell lines have been genotyped: U937 cells are homozygous slow acetylators (S1a/S1a) and HepG2 cells are heterozygous slow acetylators (S1a/S2). Transcription of pnat was confirmed in the hepatoma cell lines, by amplification of cDNA generated from these cells. In addition, splicing of mRNA specific for pNAT has been demonstrated by using a primer which anneals to a region in the 5′ promoter region. Unlike the hepatoma cell lines, in U937 cells the pNAT gene is not transcribed. However, transcription of mnat was shown to occur in all three cell lines." @default.
- W53008384 created "2016-06-24" @default.
- W53008384 creator A5069113012 @default.
- W53008384 creator A5071196257 @default.
- W53008384 date "1993-09-01" @default.
- W53008384 modified "2023-09-26" @default.
- W53008384 title "Arylamine N-acetyltransferase activity in human cultured cell lines" @default.
- W53008384 cites W1516667778 @default.
- W53008384 cites W1522302756 @default.
- W53008384 cites W1528082070 @default.
- W53008384 cites W1587924678 @default.
- W53008384 cites W1603613420 @default.
- W53008384 cites W1976246694 @default.
- W53008384 cites W1979343866 @default.
- W53008384 cites W1980571658 @default.
- W53008384 cites W2020261871 @default.
- W53008384 cites W2053454422 @default.
- W53008384 cites W2055786949 @default.
- W53008384 cites W2060333964 @default.
- W53008384 cites W2066710210 @default.
- W53008384 cites W2074457276 @default.
- W53008384 cites W2077535572 @default.
- W53008384 cites W2084397691 @default.
- W53008384 cites W2099073633 @default.
- W53008384 cites W2112351457 @default.
- W53008384 cites W2122656085 @default.
- W53008384 cites W2153651410 @default.
- W53008384 cites W2175033967 @default.
- W53008384 cites W2221470806 @default.
- W53008384 cites W2339157224 @default.
- W53008384 cites W2415326388 @default.
- W53008384 cites W2436568872 @default.
- W53008384 cites W2443603457 @default.
- W53008384 cites W4211002175 @default.
- W53008384 doi "https://doi.org/10.1042/bj2940481" @default.
- W53008384 hasPubMedCentralId "https://www.ncbi.nlm.nih.gov/pmc/articles/1134479" @default.
- W53008384 hasPubMedId "https://pubmed.ncbi.nlm.nih.gov/8396914" @default.
- W53008384 hasPublicationYear "1993" @default.
- W53008384 type Work @default.
- W53008384 sameAs 53008384 @default.
- W53008384 citedByCount "19" @default.
- W53008384 countsByYear W530083842017 @default.
- W53008384 countsByYear W530083842018 @default.
- W53008384 crossrefType "journal-article" @default.
- W53008384 hasAuthorship W53008384A5069113012 @default.
- W53008384 hasAuthorship W53008384A5071196257 @default.
- W53008384 hasBestOaLocation W530083842 @default.
- W53008384 hasConcept C101762097 @default.
- W53008384 hasConcept C104317684 @default.
- W53008384 hasConcept C119157956 @default.
- W53008384 hasConcept C150194340 @default.
- W53008384 hasConcept C153911025 @default.
- W53008384 hasConcept C176944494 @default.
- W53008384 hasConcept C2778328694 @default.
- W53008384 hasConcept C2780730277 @default.
- W53008384 hasConcept C501734568 @default.
- W53008384 hasConcept C54355233 @default.
- W53008384 hasConcept C55493867 @default.
- W53008384 hasConcept C81885089 @default.
- W53008384 hasConcept C86803240 @default.
- W53008384 hasConceptScore W53008384C101762097 @default.
- W53008384 hasConceptScore W53008384C104317684 @default.
- W53008384 hasConceptScore W53008384C119157956 @default.
- W53008384 hasConceptScore W53008384C150194340 @default.
- W53008384 hasConceptScore W53008384C153911025 @default.
- W53008384 hasConceptScore W53008384C176944494 @default.
- W53008384 hasConceptScore W53008384C2778328694 @default.
- W53008384 hasConceptScore W53008384C2780730277 @default.
- W53008384 hasConceptScore W53008384C501734568 @default.
- W53008384 hasConceptScore W53008384C54355233 @default.
- W53008384 hasConceptScore W53008384C55493867 @default.
- W53008384 hasConceptScore W53008384C81885089 @default.
- W53008384 hasConceptScore W53008384C86803240 @default.
- W53008384 hasLocation W530083841 @default.
- W53008384 hasLocation W530083842 @default.
- W53008384 hasLocation W530083843 @default.
- W53008384 hasLocation W530083844 @default.
- W53008384 hasOpenAccess W53008384 @default.
- W53008384 hasPrimaryLocation W530083841 @default.
- W53008384 hasRelatedWork W1914642345 @default.
- W53008384 hasRelatedWork W1948932778 @default.
- W53008384 hasRelatedWork W2021814587 @default.
- W53008384 hasRelatedWork W2055739858 @default.
- W53008384 hasRelatedWork W2069913570 @default.
- W53008384 hasRelatedWork W2083531614 @default.
- W53008384 hasRelatedWork W2118375848 @default.
- W53008384 hasRelatedWork W2142280385 @default.
- W53008384 hasRelatedWork W4244155605 @default.
- W53008384 hasRelatedWork W53008384 @default.
- W53008384 isParatext "false" @default.
- W53008384 isRetracted "false" @default.
- W53008384 magId "53008384" @default.
- W53008384 workType "article" @default.