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- W53054881 abstract "Recently a new sequencer cartridge designed specifically for polyvinylidene difluoride (PVDF) membranes has become commercially available (Blott cartridge, Applied Biosystems). This reaction cartridge orients the sample so that solvent flow is parallel to the protein loaded membrane surface. This orientation together with the narrow width of the sample chamber slot minimizes bubble trapping on top of the membrane and channeling of solvent around the sample is effectively eliminated or minimized. As fluid movement relative to the membranes has been improved, wetting of the membrane should be improved, facilitating better extraction of the ATZ amino acids and byproducts. This chapter evaluates this new cartridge design using a program that had already been optimized for high sensitivity sequencing on PVDF membranes to determine whether the Blott cartridge would offer any further advantages. The Blott cartridge is superior to the standard cartridge design when sequencing proteins absorbed to PVDF membranes as indicated by decreased lag or carryover and by improved tryptophan yields. Better solvent extraction reduces byproducts and/or solvent washes can be reduced. Improved solvent extraction also means that the protein side of electroblotted PVDF samples can be oriented in any direction and bands can be stacked if necessary to further increase sample loads. As more PVDF bands can be loaded, the amount of protein available for sequencing can be increased when working with very large proteins or diffuse bands." @default.
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- W53054881 date "1992-01-01" @default.
- W53054881 modified "2023-09-26" @default.
- W53054881 title "Evaluation of the Blott Cartridge for Enhanced Gas Phase Sequencing at Maximum Sensitivity" @default.
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- W53054881 doi "https://doi.org/10.1016/b978-0-12-058756-8.50011-0" @default.
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