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- W53166045 abstract "Abstract Marine environment rich in prokary otes and eukary-otes is a reservoir of novel bioactive molecules withwide chemical diversity. For this study, deep seaorganisms were collected by dredging and trawlingduring FORV Sagar Sampada Cruise # 291 Leg I andCruise # 305. Around 60 different marine organismswere sampled and screened for antimicrobial com-pounds and proteins against an array of humanpathogens such as Klebsiella pneumoniae, Bacilluscereus, Salmonella typhimurium, Escherichia coli, Sta-phylococcus aureus, Pseudomonas aeruginosa and Vibriocholerae by agar well diffusion method and discdiffusion method. Solvents such as ethanol, metha-nol, chloroform, butanol and 5% acetic acid wereused. Thin lay er chromatography and bioautographyassays of bioactiv e extracts confirmed the presence ofantimicrobial compounds in them. The separation ofthe compound was done using cyclograph. Ray fish,star fish, Loligo sp., spider crab, Maxmulleria sp. andmussel showed presence of an antimicrobial proteinthat was purified by TLC, cation exchange chroma-tography and tricine-SDS PAGE.Keywords: Antimicrobial activity, pathogens, discdiffusion, bioautography, TLC, SDS – PAGE" @default.
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- W53166045 date "2014-01-01" @default.
- W53166045 modified "2023-09-27" @default.
- W53166045 title "Bioactive Potential of Proteins from Deep Sea Organisms" @default.
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