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- W53278814 abstract "Otitis media (OM) may lead to inner ear inflammation resulting in sensorineural hearing loss. Here, we aim to determine the role of spiral ligament fibrocytes (SLFs)-derived CCL2 and exogenous IL-10 in inner ear inflammation, secondary to nontypeable H. influenzae (NTHI)-induced OM. THP-1 cells actively migrated in response to the conditioned medium of NTHI-exposed SLFs. This migratory activity was inhibited by the CC chemokine inhibitor and by the deficiency of CCL2 in SLFs. The CCR2 inhibitor and CCR2 deficiency inhibited migration of monocyte-like cells of the splenocytes in response to NTHI-induced SLF-derived CCL2. qRT-PCR showed that recombinant IL-10 inhibits SLF's NTHI-induced up-regulation of CCL2 expression. Luciferase assays showed that silencing of IL-10RA in SLFs suppressed the inhibitory effect of IL-10 on NTHI-induced CCL2 up-regulation. In contrast, IL-10 deficiency of SLFs hardly affected NTHI-induced CCL2 expression. Transtympanic inoculation of live NTHI led to inner ear inflammation in 10 out of 14 murine ears (71%). Unexpectedly, CCL2 deficiency equivocally decreased the incidence of OM-induced inner ear inflammation (60%), indicating a compensatory effect of other chemokines in vivo. Taken together, we suggest that NTHI-induced SLF-derived CCL2 contributes to OM-induced inner ear inflammation, which is negatively regulated by IL-10. [Supported in part by DC008696 and DC006276]" @default.
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- W53278814 date "2010-04-01" @default.
- W53278814 modified "2023-10-18" @default.
- W53278814 title "Yin and Yang: CCL2 and IL‐10 in Otitis Media‐induced Inner Ear Inflammation" @default.
- W53278814 doi "https://doi.org/10.1096/fasebj.24.1_supplement.952.4" @default.
- W53278814 hasPublicationYear "2010" @default.
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