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- W5330173 abstract "Infected wounds are a major cause of hospital-acquired infections and theseare difficult to treat due to the emergence of antibiotic-resistant bacteria. Thisproject is concerned with evaluating a novel antimicrobial approach involvingthe photosensitizer indocyanine green (ICG) which generates reactive oxygenspecies when irradiated with near-infrared (NIR) light which enables goodtissue penetration. The photo-susceptibility of common wound-infectingorganisms to ICG coupled with NIR-light was investigated. All species weresusceptible to killing. ICG at a concentration of 25 μg/mL enabled the killing ofthe Gram-positive species (Staphylococcus aureus and Streptococcuspyogenes), higher concentrations (100-200μg/mL) were necessary to achievesubstantial kills of the Gram-negative species (Pseudomonas aeruginosa andEscherichia coli). Both high and low fluences were able to kill 99.999% of theGram-positive bacteria. High fluence irradiation was necessary to kill 99.99%of the Gram-negative bacteria. The pulsed-mode of irradiation was as effectiveas the continuous-mode for killing the Gram-positive species. Yet only thecontinuous-mode of irradiation was able to kill P. aeruginosa. Biofilms ofStaph. aureus and P. aeruginosa were susceptible to disruption and killing byICG-photosensitization. A significant enhancement of lethal photosensitizationof Staph. aureus was achievable using gold-nanoparticles and antioxidants.Significant kills (>99%) were achieved in the presence of serum and 100 μg/mLICG. A low oxygen concentration reduced the kills to 96.77% and 71.62% forStaph. aureus and Strep. pyogenes respectively. Mechanistic studiesrevealed that killing was mediated mainly by reactive-oxygen species.In vivo studies in mice showed that ICG and continuous-NIR light could achievekills of 96%, 93% and 78-91% for P. aeruginosa, Strep. pyogenes and Staph.aureus respectively.The results of these in vitro and in vivo studies imply that ICG-PDT could be aneffective means of decreasing the microbial burden in wounds." @default.
- W5330173 created "2016-06-24" @default.
- W5330173 creator A5026483842 @default.
- W5330173 date "2010-02-28" @default.
- W5330173 modified "2023-09-24" @default.
- W5330173 title "Killing of Organisms Responsible for Wound Infections Using a Light-activated Antimicrobial Agent" @default.
- W5330173 hasPublicationYear "2010" @default.
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