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- W53512212 abstract "In maize the transposable elements Activator/Dissociation (Ac/Ds) transpose shortly afterreplication from one of the two resulting chromatids (chromatid selectivity). A modelwas suggested that explains this phenomenon as a consequence of different Ac transposasebinding to holo-, hemi- and unmethylated transposon ends (Wang et al., 1996). It assumesthat before replication the element is holomethylated and does not transpose because TPasecan not bind to the transposon ends. Shortly after replication one of the two differentiallyhemimethylated daughter transposons should become transposition competent.However, DNA methylation-mediated replication dependence does not completely explainthe behavior of Ac/Ds transposition. In several studies performed in monocot hosts, it wasfound that in a transient assay Ac/Ds element excision from extrachromosomal geminivirusvectors occurs only during vector replication, although the transfected DNAs were not Cmethylated.In this work the correlation between Ds transposition, DNA replication and DNAmethylation in the dicot species Petunia hybrida was studied. Ds reporter vectorsharboring TYLCV (Tomato Yellow Leaf Curl Virus) geminivirus replicon sequences andreplicating in transfected petunia cells were constructed. It has been shown that thetransposition of a Ds element from an extrachromosomal vector in petunia cells isregulated by DNA replication in a methylation-dependent and -independent mode.Holomethylation completely inhibits Ds excision from a non-replicating plasmid, whereasDs transposition is restored by replication. Moreover, Ds elements that are hemimethylatedon one DNA strand transpose in the absence of replication, whereas methylation on thecomplementary DNA strand results in at least 6.3-fold reduced excision frequencies. Thesedata strongly support the transposition model of Wang et al. (1996).Beyond that, Ds transposition is strongly promoted by replication also in the absence ofmethylation. It has been shown that in petunia cells, unlike monocot hosts, replication isnot a prerequisite for Ds transposition, nevertheless it enhances Ds transposition by at leasta factor of 7.5. Moreover, replication promotes the formation of a predominant excisionfootprint. Implications on the mechanism and regulation of Ac/Ds transposition arediscussed." @default.
- W53512212 created "2016-06-24" @default.
- W53512212 creator A5068715727 @default.
- W53512212 date "2001-03-09" @default.
- W53512212 modified "2023-09-26" @default.
- W53512212 title "Regulation of maize Ac/Ds transposition by replication and DNA methylation" @default.
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