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- W53595053 abstract "Publisher Summary This chapter describes the isolation procedures for obtaining mitochondrial DNA (mtDNA) from three tissue types of several genera of dicot plants. It discusses the isolation of suspension culture mitochondria capable of active in organello protein synthesis. Alternative procedures, including analysis of mtDNAs present in total DNA preparations, dye-mediated gradient separations of organelle DNAs, and use of other tissue sources are also discussed. The key criterion for a successful mitochondrial preparation is freedom from contaminating plastid and nuclear DNAs and plastid and cytoplasmic ribosomes. Significant contamination with other subcellular particles can be tolerated provided these do not contain nucleic acids or ribosomes capable of protein synthesis. A key feature of mitochondrial isolation procedures is differential centrifugation to separate the bulk of nuclei, plastids, and cellular debris from the mitochondrial fraction that differs in particle size or density." @default.
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- W53595053 date "1986-01-01" @default.
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- W53595053 title "The isolation of mitochondria and mitochondrial DNA" @default.
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