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- W53877878 abstract "This chapter describes the thymus as a vehicle for lymphoid differentiation and the status of thymus epithelial cultures and T-cell differentiation. A number of protocols designed to stimulate epidermal cells and co-culture with irradiated fibroblasts have uniformly failed to stimulate thymus epithelial growth. Whole organ culture, therefore, appears to contain epithelial cells only in the residue of the explant. Very similar results have been obtained with trypsinized, and mechanically disrupted thymuses. By serial transfers and addition of murine amniotic fluid to the culture medium, it has been possible to grow only the macrophage population from organ expiants although cell lines of these have not yet been obtained. Ultrastructural and light microscope studies have shown firstly that ciliated cells are constantly observed in fetal thymus organ culture, associated with vacuolations, or cysts, and that an electron-dense cell coat covers the outer surfaces of epithelial cells but is absent from the ciliated cells." @default.
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- W53877878 date "1981-01-01" @default.
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- W53877878 title "THE THYMUS AS A VEHICLE FOR LYMPHOID DIFFERENTIATION; THE STATUS OF THYMUS EPITHELIAL CULTURES AND T-CELL DIFFERENTIATION" @default.
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- W53877878 doi "https://doi.org/10.1016/b978-0-08-025922-2.50105-4" @default.
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