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- W53939952 abstract "Frozen, imported raspberries have been linked to several outbreaks of humannorovirus (NoV) gastroenteritis in Sweden. Noroviruses are highly infectious andare often presented in low numbers in contaminated foods. Detection with RT-PCRmust therefore be preceded by a method that extract and concentrate viruses fromthe tested food samples, but most available methods are laborious and/or inefficient.Studies on noroviruses are further complicated by the fact that human norovirusesare unable to grow in routinely used cell culture models. The murine norovirus(MNV), a common pathogen of immunocompromised mice, can be cultivated inmacrophage-like cells and is often used as a model for studies on humannoroviruses.In the present study, a previously unidentified MNV isolate from Sweden wassequenced and molecularly characterized. This isolate also served as a surrogate fortesting and further developing a method to concentrate noroviruses from raspberries.Pathatrix (Life Technologies) is an automated magnet separation system thatallows concentration of viruses or bacteria from large sample volumes. This systemwas tested together with cationic paramagnetic beads that attract the negativelycharged surface of NoV particles. Results from this study shows that few modificationsof the Pathatrix protocol might enhance viral recovery. For further evaluation,the Pathatrix method was compared with polyethylene glycol (PEG) precipitationfor concentrating MNV, NoV GI, and GII from artificially contaminated raspberries.PEG precipitation was clearly more efficient but displayed a high degree ofinhibition in RT-PCR.Altogether, this study shows that the Pathatrix method is a convenient and quickalternative. However, it needs to be further optimized before it can be used to concentratenoroviruses from raspberries." @default.
- W53939952 created "2016-06-24" @default.
- W53939952 creator A5028630101 @default.
- W53939952 date "2013-02-15" @default.
- W53939952 modified "2023-09-24" @default.
- W53939952 title "Molecular characterization of a murine norovirus isolate from Sweden and detection of noroviruses in artificially contaminated raspberries" @default.
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