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- W5413109 abstract "ABSTRACT The presence of budding C-type and intracytoplasmic A-type particles in Chinese hamster ovary (CHO) cells is well documented. However, extensive screening has failed to detect any evidence of infectivity. We have used a high-capacity, continuous-flow, ultracentrifuge rotor to concentrate extracellular particles from culture fluid of recombinant CHO cells for molecular characterization. Equilibrium sucrose gradient sedimentation of particle preparations results in co-fractionation of reverse transcriptase activity and mammalian C-type retrovirus core proteins at a density characteristic of retrovirus particles. Electron microscope examination of gradient fractions at this density revealed the presence of particles with morphology and size similar to other characterized retroviruses. Several cDNA clones of particle RNA homologous to the endonuclease region of Moloney murine leukemia virus have been isolated and sequenced. None exhibit open reading frames capable of encoding an intact endonuclease, providing one possible explanation for the non-infectious nature of the observed particles. The presence of conserved C-type provirus sequences in the DNA of all CHO cell lines examined, as well as in Chinese hamster liver DNA, suggests that the observed particles are the products of endogenous retroviruslike elements present in the germline of Chinese hamsters. Keywords: CHO cells, C-type particles, intracisternal A-particles, endogenous retrovirus, endonuclease, reverse transcriptase, core protein, cDNA, continuous-flow ultracentrifugation, sucrose gradients." @default.
- W5413109 created "2016-06-24" @default.
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- W5413109 date "1991-01-01" @default.
- W5413109 modified "2023-09-23" @default.
- W5413109 title "DEFECTIVE ENDOGENOUS RETROVIRUSLIKE PARTICLES OF CHINESE HAMSTER OVARY CELLS" @default.
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- W5413109 doi "https://doi.org/10.1016/b978-0-7506-1103-9.50014-7" @default.
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