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• W54156420 abstract "Long-term culture of mesenchymal stem cells (MSC) has major impact on cellular characteristics and differentiation potential. Numerous clinical trials raise high hopes in regenerative medicine and this necessitates reliable quality control of the cellular products—also with regard to replicative senescence. The maximum number of population doublings before entering the senescent state depends on the cell type, tissue of origin, culture medium as well as cell culture methods. Therefore, it would be valuable to predict the remaining proliferative potential in the course of culture expansion. Here, we describe a refined fibroblastic colony forming unit (CFU-f) assay which can be performed at any passage during culture expansion with simple cell culture techniques. This method is based on limiting dilutions in the 96-well format to determine the proportion of highly proliferative and clonogenic cells. The number of CFU-f declines rapidly during culture expansion. Especially at higher passages the CFU-f frequency correlates very well with the remaining cumulative population doublings. This approach can be used as quality measure to estimate the remaining proliferative potential of MSC in culture." @default.
• W54156420 created "2016-06-24" @default.
• W54156420 creator A5021198353 @default.
• W54156420 creator A5026397452 @default.
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• W54156420 date "2013-01-01" @default.
• W54156420 modified "2023-09-26" @default.
• W54156420 title "Tracking of Replicative Senescence in Mesenchymal Stem Cells by Colony-Forming Unit Frequency" @default.
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• W54156420 doi "https://doi.org/10.1007/978-1-62703-317-6_11" @default.
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